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Anti-HA Tag Antibody
基本信息
eCl@ss | 32160702 |
NACRES | NA.43 |
General description【一般描述】 | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. |
Specificity【特异性】 | Recognizes HA tagged proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells. Reactivity with other species has not been confirmed. |
Immunogen【免疫原】 | KLH-conjugated, synthetic peptide corresponding to amino acids 98-106 of influenza hemaglutinin. |
Application【应用】 | Immunoprecipitation: 5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein. Immunocytochemistry: 5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein. Detect HA Tag using this Anti-HA Tag Antibody validated for use in IC, IP & WB. Research Category Epitope Tags & General Use Research Sub Category Epitope Tags |
Quality【质量】 | Routinely evaluated by western blot of recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells. Western Blot Analysis: 0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells. |
Linkage【联系】 | Replaces: 07-221 |
Physical form【外形】 | Format: Purified Protein A purified Purified mouse monoclonal IgG3 in buffer containing PBS, pH 7.4 containing 0.05% sodium azide. |
Analysis Note【分析说明】 | Control RIPA lysates from HEK293 cells. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | monoclonal |
species reactivity | vertebrates (common species) |
manufacturer/tradename | Upstate® |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG3 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | Various |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |