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Anti-EED Antibody, clone AA19

产品编号:4084633
规格:ascites fluid, from mouse
包装规格:100 μg
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4084633100 μg6690
产品别名

Anti-EED Antibody, clone AA19

WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. EED is also known to bind H3K27me3 and can allosterically stimulate the methyltransferase activity of the PRC2 complex. (Ref.: Hojfeldt, JW. et al (2018) Nat. Struc. Mol. Biol. 25 (3); 225-232).
Specificity【特异性】
Not tested in other species.
This antibody recognizes mouse EED, (~30-65 kDa).
Immunogen【免疫原】
Recombinant fusion protein of full length murine EED tagged with MBP.
Application【应用】
Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer
Research Sub Category
Chromatin Biology
Anti-EED Antibody, clone AA19 is a mouse monoclonal antibody for detection of EED also known as WD protein associating with integrin cytoplasmic tails 1 ,embryonic ectoderm development & has been validated in WB.
Quality【质量】
Routinely evaluated by Western Blot on A431cel lysatel.

Western Blot Analysis: 1:5,000 of this lot detected EED on 15 μg/mL of A431 cell lysate.

Physical form【外形】
Purified immunoglobulin in 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
Analysis Note【分析说明】
Control
A431 Cell Lysate
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
ascites fluid
antibody product type
primary antibodies
clone【克隆】
monoclonal
species reactivity
human, mouse
technique(s)
western blot: suitable
isotype【同位素/亚型】
IgG2aκ
NCBI accession no.【NCBI登记号】
NP_068676
UniProt accession no.【UniProt登记号】
O75530
产品说明
Target description【目标描述】
Ranges from 30-65 kDa (5-6 isoforms of EED are detected).
Storage and Stability【储存及稳定性】
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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