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Anti-EED Antibody, clone AA19
产品别名
Anti-EED Antibody, clone AA19
WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. EED is also known to bind H3K27me3 and can allosterically stimulate the methyltransferase activity of the PRC2 complex. (Ref.: Hojfeldt, JW. et al (2018) Nat. Struc. Mol. Biol. 25 (3); 225-232). |
Specificity【特异性】 | Not tested in other species. This antibody recognizes mouse EED, (~30-65 kDa). |
Immunogen【免疫原】 | Recombinant fusion protein of full length murine EED tagged with MBP. |
Application【应用】 | Research Category Epigenetics & Nuclear Function Apoptosis & Cancer Research Sub Category Chromatin Biology Anti-EED Antibody, clone AA19 is a mouse monoclonal antibody for detection of EED also known as WD protein associating with integrin cytoplasmic tails 1 ,embryonic ectoderm development & has been validated in WB. |
Quality【质量】 | Routinely evaluated by Western Blot on A431cel lysatel. Western Blot Analysis: 1:5,000 of this lot detected EED on 15 μg/mL of A431 cell lysate. |
Physical form【外形】 | Purified immunoglobulin in 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide. Protein G Purified |
Analysis Note【分析说明】 | Control A431 Cell Lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | ascites fluid |
antibody product type | primary antibodies |
clone【克隆】 | monoclonal |
species reactivity | human, mouse |
technique(s) | western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_068676 |
UniProt accession no.【UniProt登记号】 | O75530 |
产品说明
Target description【目标描述】 | Ranges from 30-65 kDa (5-6 isoforms of EED are detected). |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |