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Anti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303
产品别名
Anti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303
H3K4me2, Histone H3 (di methyl K4), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Dimethyl-lysine 4 histone H3 (H3K4me2) is a transcription-activating chromatin mark, and dimethyl histone H3 Lys4 (H3K4me2) is depleted from regions with DNA methylation. Multipotential hematopoietic cells have a subset of genes that are differentially methylated (H3K4me2+/me3-). These genes are transcriptionally silent and highly enriched in lineage-specific hematopoietic genes, suggesting a role for H3K4 methylation in differentiation. |
Specificity【特异性】 | This antibody specifically recognizes Histone H3 dimethylated at Lys4. The antibody binding specificity allows for phosphorylation of Thr3 and/or modifications of Lys91. Broad species cross-reactivity is expected, based on sequence homology. |
Immunogen【免疫原】 | Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, dimethylated on Lys4, conjugated to KLH. Epitope: Dimethylated Lys4 |
Application【应用】 | Research Sub Category Histones Research Category Epigenetics & Nuclear Function Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG, or Anti-dimethyl-Histone H3 (Lys4) antibody and the Magna ChIP G (Cat. # 17-611) Kit. Successful immunoprecipitation of dimethyl-histone H3 (Lys4) associated DNA fragments was verified by qPCR using ChIP Primers GAPDH Coding. Please refer to the EZ-Magna G ChIP Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 05-1338, Anti-dimethyl H3 (Lys4), clone CMA303 at 2.0 µg/mL (1:500 dilution). Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system. ChIP-seq Analysis: Chromatin immunoprecipitation was performed using the Magna ChIP Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry. Immunoprecipitation: This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation. ELISA: This antibody has been shown by an outside laboratory to be suitable for ELISA Multiplexing: This antibody specifically recognizes histone H3 dimethylated on Lys4 by Luminex Anti-Dimethyl Histone H3 (Lys4) Antibody, clone CMA303 is a mouse monoclonal antibody for detection of Dimethyl Histone H3 (Lys4) also known as H3K4me2, Histone H3 (di methyl K4). Demonstrated performance in ChIP, DB, ELISA, ICC, IP, WB, Mplex. |
Quality【质量】 | Routinely evaluated by Western Blot on HeLa acid extract. Western Blot Analysis: 2 μg/mL (1:500) dilution of this antibody detected dimethyl Histone H3 (Lys4) on 10 μg of HeLa acid extract. |
Physical form【外形】 | Purified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide. Format: Purified Protein G Purified |
Analysis Note【分析说明】 | Control HeLa Acid extract lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | CMA303, monoclonal |
species reactivity | vertebrates, human |
technique(s) | ChIP: suitable ELISA: suitable dot blot: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable multiplexing: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1κ |
NCBI accession no.【NCBI登记号】 | NM_003493 |
UniProt accession no.【UniProt登记号】 | Q16695 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | approx. 17 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |