InSolution Calyculin A, Discodermia calyx-CAS 101932-71-2-Calbiochem

C₅₀H₈₁N₄O₁₅P

1009.17

MFCD06795864

Cell-permeable protein phosphatase 2A (PP2A) inhibitor (IC₅₀ = 0.5-1.0 nM). Also inhibits protein phosphatase 1 (PP1; IC₅₀ = 2 nM). A non-phorbol ester type tumor promoter that induces oxidative DNA damage. Selectively increases the efficacy of AMPA receptor-mediated synaptic transmission at excitatory synapses. Recently, it has been used to study the regulation of Ca²⁺-activated K⁺ channels in smooth muscle and to correlate shape changes in fibroblasts with the degree of protein phosphorylation. Also known to prevent γ-radiation induced apoptosis in Burkitt′s lymphoma cell line BM13674.

Cell permeable: yes
Target IC₅₀: 0.5-1.0 and 2 nM for PP2A and PP1, respectively
Reversible: yes
Primary Target
protein phosphatase-2A & -1

Toxicity: Standard Handling (A)

A 100 µM (5 µg/50 µL) sterile-filtered solution of Calyculin A, Discodermia calyx (Cat. No. 208851) in DMSO.

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C. Use only fresh DMSO for further dilutions.

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Gopalakrishna, R., et al. 1992. Biochem. Biophys. Res. Commun.189, 950.
Carl, A., et al. 1991. Am. J. Physiol.261, C387.
Suganuma, M., et al. 1990. Cancer Res.50, 3521.
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Cohen, P., et al. 1989. J. Biol. Chem.264, 21435.
Ishihara, H., et al. 1989. Biochem. Biophys. Res. Commun.159, 871.
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Kato, Y., et al. 1986. J. Am. Chem. Soc.108, 2780.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

liquid

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles
desiccated (hygroscopic)
protect from light

−70℃

1S/C50H81N4O15P/c1-29(20-22-51)16-14-17-30(2)32(4)24-33(5)42(57)35(7)38(55)25-41(65-13)45-46(69-70(61,62)63)49(8,9)50(68-45)26-39(56)34(6)40(67-50)19-15-18-36-27-66-48(53-36)31(3)21-23-52-47(60)44(59)43(58)37(28-64-12)54(10)11/h14-18,20,24,27,31,33-35,37-46,55-59H,19,21,23,25-26,28H2,1-13H3,(H,52,60)(H2,61,62,63)/b16-14+,18-15+,29-20-,30-17+,32-24+/t31-,33+,34+,35+,37-,38-,39-,40+,41-,42+,43-,44-,45+,46-,50-/m0/s1

FKAWLXNLHHIHLA-QJLNTFFJSA-N

Packaged under inert gas

10 - Combustible liquids

WGK 1

188.6 °F - (refers to pure substance)

87 ℃ - (refers to pure substance)