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Anti-MYO1C Antibody, clone 4B19, ZooMAb® Rabbit Monoclonal
产品编号: | 4083135 |
规格: | recombinant, expressed in HEK 293 cells |
包装规格: | 25 μL,4 X 25 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-MYO1C Antibody, clone 4B19, ZooMAb® Rabbit Monoclonal
MMIb
MMI-beta
Unconventional myosin-Ic
Myosin I beta
产品性质
biological source【生物来源】 | rabbit (recombinant) |
recombinant【重组】 | expressed in HEK 293 cells |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 4B19, monoclonal recombinant monoclonal |
product line【产品线】 | ZooMAb® learn more |
form【形式】 | lyophilized |
mol wt【分子量】 | calculated mol wt 119.63 kDa observed mol wt ~120 kDa |
species reactivity | human, mouse |
species reactivity (predicted by homology) | porcine |
packaging【包装】 | pkg of 25 μL |
greener alternative product characteristics【环保替代产品特性】 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
enhanced validation【增强验证】 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG |
greener alternative category【环保替代产品分类】 | Aligned |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | 2-8℃ |
基本信息
General description【一般描述】 | ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
Specificity【特异性】 | Clone 4B19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Unconventional myosin-Ic. It targets an epitope within 16 amino acids from the N-terminal region. May also display reactivity with isoform 1. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to the first 16 amino acids from human Unconventional myosin-Ic, isoform 3. |
Application【应用】 | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MYO1C in HT29, HEK293, and NIH3T3 cell lysates. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MYO1C in human stomach and human tonsil tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MYO1C in HeLa, A431, HUVEC, NIH 3T3, and HT-29 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. Anti-MYO1C, clone 4B19 ZooMAb, Cat. No. ZRB1372, , a recombinant Rabbit monoclonal antibody that targets Unconventional myosin-Ic (MYO1C) and is tested in Immunocytochemistry and Immunohistochemistry, and Western Blotting. |
Physical form【外形】 | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
Reconstitution【重悬】 | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
Legal Information【法律信息】 | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品说明
Target description【目标描述】 | Unconventional myosin-Ic (UniProt: O00159; also known as Myosin I beta, MMI-beta, MMIb, MYO1C) is encoded by the MYO1C gene (Gene ID: 4641) in human. Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Myosins form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments. MYO1C, a single-headed class I myosin, localized to the cell surface. It is associated with cholesterol-enriched lipid rafts and enhances their recycling from intracellular compartments to the cell surface. It is involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. It is reported to colocalize with RNA polymerase II in the nucleus and with RNA polymerase I in nucleoli. In the nucleolus, it is localized predominantly in the dense fibrillar component (DFC) and in granular component. The MYO1C gene produces three alternatively spliced isoforms that differ only in their N-terminal regions. Although they share identical motor domains, three calmodulin-binding IQ motifs containing a nuclear localization signal, and a membrane-binding tail domain, each isoform has different function and nuclear cytoplasm partitioning. These isoforms display both specific and overlapping nuclear and cytoplasmic functions and have different expression levels. Isoform 3 of MYO1C is involved in regulation of transcription and associates with transcriptional active ribosomal genes and cooperates with the WICH chromatin-remodeling complex to facilitate transcription. It is shown to be essential for the formation of the first phosphodiester bond during transcription initiation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zattelman L., et al. (2017). J Biol Chem. 292(43);17804-17818). |
Storage and Stability【储存及稳定性】 | Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |