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Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, ZooMAb® Rabbit Monoclonal

产品编号:4082934
规格:recombinant, expressed in HEK 293 cells
包装规格:25 μL,4 X 25 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
408293425 μL2350
40829344 X 25 μL4400
产品别名

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, ZooMAb® Rabbit Monoclonal

Trimethylated Histone H3

Histone H3

Histone H3Kme3

产品性质
biological source【生物来源】
rabbit (recombinant)
recombinant【重组】
expressed in HEK 293 cells
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
MC315, monoclonal
recombinant monoclonal
product line【产品线】
ZooMAb® learn more
form【形式】
lyophilized
mol wt【分子量】
calculated mol wt 15.51 kDa
observed mol wt ~17 kDa
species reactivity
human, mouse
packaging【包装】
antibody small pack of 25 μL
greener alternative product characteristics【环保替代产品特性】
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation【增强验证】
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunocytochemistry: suitable using 1:100
immunohistochemistry: suitable using 1:100
inhibition assay: suitable using 1:250 (peptide)
western blot: 1:250-1:1,000
isotype【同位素/亚型】
IgG
UniProt accession no.【UniProt登记号】
Q16695
greener alternative category【环保替代产品分类】
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
Gene Information
human ... HIST3H3;H3FT(8290)
基本信息
General description【一般描述】
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.
Specificity【特异性】
Clone MC315 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Histone H3 trimethylated on Lysine 4.
Immunogen【免疫原】
BSA-conjugated linear peptide corresponding to an amino acid sequence containing (RTK4me3Q) (trimethylated lysine 4) from human Histone H3.
Application【应用】
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in human colon and human breast cancer tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in A431, HeLa, HUVEC, and NIH 3T3 cells.

Peptide Inhibition Analysis: A 1:250 dilution from a representative lot was used with HeLa acid extract for peptide block analysis.
Anti-trimethyl-Histone H3 (Lys4), clone MC315 ZooMAb, Cat. No. ZRB04791, is a highly specific rabbit recombinant monoclonal antibody that targets Histone H3 trimethylatled on lysine 4 and has been tested for use in Immunocytochemistry, Immunohistochemistry, Peptide Inhibition Assay, & Western Blot.
Physical form【外形】
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution【重悬】
30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Legal Information【法律信息】
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
产品说明
Target description【目标描述】
Histone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. Histone H3 contains a main globular domain and a long N-terminal tail and is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. H3K4me3 modifications are reported to occur consistently at transcription start sites and H3K4me3 domain is associated with higher transcription activity and cell identity in pre-implantation development and in the process of deriving embryonic stem cells from the inner cell mass and trophoblast stem cells from the trophectoderm. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liu, X., et al. (2016). Nature 537(7621); 558-562).
Storage and Stability【储存及稳定性】
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 1
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