您的位置:首页 > 产品中心 > Anti-Talin-1 Antibody, clone 2F8 ZooMAb® Rabbit Monoclonal
Anti-Talin-1 Antibody, clone 2F8 ZooMAb® Rabbit Monoclonal
产品编号: | 4082927 |
规格: | recombinant, expressed in HEK 293 cells |
包装规格: | 25 μL,4 X 25 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
recombinant【重组】 | expressed in HEK 293 cells |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 2F8, recombinant monoclonal |
description【描述】 | recombinant, expressed in HEK 293 cells |
product line【产品线】 | ZooMAb® learn more |
form【形式】 | lyophilized |
mol wt【分子量】 | calculated mol wt 269.77 kDa observed mol wt ~270 |
species reactivity | mouse, human, monkey |
packaging【包装】 | antibody small pack of 25 μL |
enhanced validation【增强验证】 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG |
epitope sequence【表位序列】 | C-terminal half |
Protein ID accession no.【Protein ID登记号】 | NP_006280 |
UniProt accession no.【UniProt登记号】 | Q9Y490 |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | 2-8℃ |
基本信息
General description【一般描述】 | ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them. Learn more about ZooMAb here. |
Specificity【特异性】 | Clone 2F8 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Talin-1. It targets an epitope within 17 amino acids from the C-terminal half. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of human Talin-1. |
Application【应用】 | Quality Control Testing Evaluated by Western Blotting in K562 cell lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Talin-1 in K562 cell lysate. Tested applications Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Talin-1 in Cos-7 cell lysate. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Talin-1 in human tonsil tissue sections. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Talin-1 in HeLa, A431, HepG2, NIH 3T3 and Jurkat cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user Anti-Talin-1, clone 2F8 ZooMAb®, Cat. No. ZRB1361, is a recombinant Rabbit monoclonal antibody that specifically detects Talin-1 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
Physical form【外形】 | Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL. |
Reconstitution【重悬】 | 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
Legal Information【法律信息】 | ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品说明
Target description【目标描述】 | Talin-1 (UniProt: Q9Y490) is encoded by the TLN1 (also known as KIAA1027, TLN) gene (Gene ID: 7094) in human. Talin-1 is a peripheral membrane protein that localized in adhesion complexes between cells and the extracellular matrix. It is shown to colocalize with layilin at the membrane ruffles and localized preferentially in focal adhesions than fibrillar adhesions. Talin-1 is involved in connections of major cytoskeletal structures to the plasma membrane. It binds to and activates integrins, directly connecting integrins to the actin cytoskeleton and recruiting numerous proteins, such as vinculin and focal adhesion kinase into integrin adhesion complexes. Talin-1 contains a globular N-terminal head region and a flexible rod domain, which can be dissociated by the action of calpain 2. The head region contains a FERM (protein 4.1, ezrin, radixin, moesin) domain that has binding sites for the cytoplasmic domains of β-integrins, layilin, and F-actin. The head region is also known to bind PIPK1 90 and the focal adhesion kinase. The rod domain is known to have an additional integrin-binding site, two actin-binding sites, and several binding sites for vinculin. Talin-1 expression is upregulated in several human cancers and accelerates tumor cell migration and metastasis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ji, L., et al. (2019). Oncol. Lett. 18(5); 5408-5416; David R. Critchley, DR., and Gingras, AR. (2008). J. Cell Sci. 121(9); 1345-1347). |
Storage and Stability【储存及稳定性】 | Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |