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Anti-Survivin Antibody, clone 1N7 ZooMAb® Rabbit Monoclonal

产品编号:4082924
规格:recombinant, expressed in HEK 293 cells
包装规格:
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4082924 2350
4082924recombinant, expressed in HEK 293 cells4400
产品别名

Anti-Survivin Antibody, clone 1N7 ZooMAb® Rabbit Monoclonal

Baculoviral IAP repeat-containing protein 5;Apoptosis inhibitor 4;Apoptosis inhibitor survivin

产品性质
Quality Level【质量水平】
200
biological source【生物来源】
rabbit
recombinant【重组】
expressed in HEK 293 cells
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
1N7, recombinant monoclonal
description【描述】
recombinant, expressed in HEK 293 cells
product line【产品线】
ZooMAb® learn more
form【形式】
lyophilized
mol wt【分子量】
calculated mol wt 16.39 kDa
observed mol wt ~16 kDa
purified by【纯化方式】
using Protein A
species reactivity
human
species reactivity (predicted by homology)
bovine, monkey, canine, feline, porcine
packaging【包装】
antibody small pack of 25 μL
greener alternative product characteristics【环保替代产品特性】
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation【增强验证】
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable
isotype【同位素/亚型】
IgG
epitope sequence【表位序列】
Internal
Protein ID accession no.【Protein ID登记号】
NP_001012270
UniProt accession no.【UniProt登记号】
O15392
greener alternative category【环保替代产品分类】
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
基本信息
General description【一般描述】
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity【特异性】
Clone 1N7 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects survivin. It targets an epitope within 21 amino acids from the internal region.
Immunogen【免疫原】
KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human Survivin.
Application【应用】
Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Survivin in Jurkat cell lysate.

Tested applications

Affinity Binding Assay: A representative lot of this antibody bound Survivin peptide with a KD of 1.7 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Survivin in MCF-7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form【外形】
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution【重悬】
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Legal Information【法律信息】
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
产品说明
Target description【目标描述】
Baculoviral IAP repeat-containing protein 5 (UniProt: O15392; also known as Apoptosis inhibitor 4, Apoptosis inhibitor survivin) is encoded by the BIRC5 (also known as API4, IAP4) gene (Gene ID: 332) in human. Survivin, a multi-tasking protein, is the smallest member of the inhibitor of apoptosis (IAP) family of proteins that inhibits apoptosis by blocking the activity of caspases. It is also involved in the regulation of cell cycle. Overexpression of survivin is reported in a large number of malignancies. Survivin is expressed highly at G2/M phase and declines rapidly in G1 phase of cell cycle. It is a component of the chromosome passage protein complex (CPC) that is shown to be essential for chromosome alignment and segregation during mitosis and cytokinesis. It acts as an important regulator of the localization of the CPC complex by directing its movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis. It also participates in the organization of the center spindle by associating with polymerized microtubules. Survivin can undergo phosphorylation at multiple sites. Phosphorylation at threonine 117 by Aurora kinase B that prevents its interaction with inner centromere protein and localization to mitotic chromosomes. Phosphorylation at threonine 48 by casein kinase 2 is shown to be important for its mitotic and anti-apoptotic activities. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.(Ref.: Jaiswal, PK., et al. (2015). Indian J. Med. Res. 141(4); 389-397; Wheatley, SP., et al. (2004). J. Biol. Chem. 279(7); 5655-5660; Li, F., et al. (1998). Nature. 396(6711); 580-584).
Storage and Stability【储存及稳定性】
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 1
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