Anti-Recoverin Antibody, clone 1K10 ZooMAb^® Rabbit Monoclonal

rabbit (recombinant)

expressed in HEK 293 cells

unconjugated

purified antibody

primary antibodies

1K10, monoclonal
recombinant monoclonal

ZooMAb^® learn more

lyophilized

calculated mol wt 23.13 kDa
observed mol wt 20 kDa

bovine, human

antibody small pack of 25 μL

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recombinant expression
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affinity binding assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

IgG

Aligned

ambient

2-8℃

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Clone 1K10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Recoverin.

His-tagged full-length human recombinant Recoverin.

Anti-Recoverin, clone 1K10 ZooMAb, Cat. No. ZRB1448, is a recombinant rabbit monoclonal antibody that specifically targets Recoverin and is tested for use in Affinity Binding Assay, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Recoverin in human retina tissue sections.

Immunofluorescence Analysis: 1:500 dilution from a representative lot detected Recoverin in human retina tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant recoverin protein with a KD of 6.0 x 10-9 in an affinity binding assay.

Immunocytochemistry Analysis: 1:100 dilution of this antibody detected Recoverin in Y79 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Recoverin (UniProt P35243; also known as Cancer-associated retinopathy antigen, Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known as RCV1) gene (Gene ID 5957) in human. The photoreceptor protein recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins. It acts as a calcium sensor and regulates phototransduction of cone and rod photoreceptor cells. It modulates light sensitivity of cone photoreceptor in dark and dim conditions. It improves rod photoreceptor sensitivity in dim light and mediates response of rod photoreceptors to facilitate detection of change and motion in bright light. In response to high Ca²⁺ levels induced by low light levels, it prolongs RHO/rhodopsin activation in rod photoreceptor cells by binding to and inhibiting G-protein-coupled receptor kinase 1 (GRK1)-mediated phosphorylation of RHO/rhodopsin. Recoverin is composed of four EF-hand domains (aa 25-60, 61-96, 97-132, 147-182), with the second and third EF-hand regions mediating low- and high-affinity calcium binding, respectively. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

11 - Combustible Solids

WGK 1