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Anti-Integrin pan-αV Antibody, clone EM01309 , ZooMAb® Rabbit Monoclonal

产品编号:4082812
规格:recombinant, expressed in HEK 293 cells
包装规格:25 μL,4 X 25 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
408281225 μL2200
40828124 X 25 μL4120
产品别名

Anti-Integrin pan-αV Antibody, clone EM01309 , ZooMAb® Rabbit Monoclonal

CD51

Vitronectin receptor

Vitronectin receptor subunit alpha

产品性质
biological source【生物来源】
rabbit (recombinant)
recombinant【重组】
expressed in HEK 293 cells
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
EM01309, recombinant monoclonal
product line【产品线】
ZooMAb® learn more
form【形式】
lyophilized
mol wt【分子量】
calculated mol wt 116.04 kDa
observed mol wt ~140 kDa
species reactivity
human
packaging【包装】
antibody small pack of 25 μL
greener alternative product characteristics【环保替代产品特性】
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation【增强验证】
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype【同位素/亚型】
IgG
greener alternative category【环保替代产品分类】
Aligned
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
基本信息
General description【一般描述】
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.
Specificity【特异性】
Clone EM01309 is a ZooMAb rabbit recombinant monoclonal antibody that detects all alphaV integrins.
Immunogen【免疫原】
Human recombinant transmembrane-truncated (DTM) extracellular domains of integrins avb3, avb5, avb6, and avb8
Application【应用】
Anti-Integrin pan-alphaV, clone EM01309, Cat. No. ZRB1189, is a recombinant Rabbit monoclonal antibody that targets integrin alphaV and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Integrin pan-alphaV in HT-29 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin pan-alphaV in HT-29 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Integrin pan-alphaV in human lung cancer and human prostate cancer tissue sections.

Flow Cytometry Analysis: 1 μg from a representative lot detected Integrin pan-alphaV in one milliion A549 cells.

Note: Actual optimal working dilutions must be determined by the end user as specimens, and experimental conditions may vary.
Physical form【外形】
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution【重悬】
300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Legal Information【法律信息】
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
产品说明
Target description【目标描述】
Integrin alphaV (UniProt: P06756; also known as Vitronectin receptor, Vitronectin receptor subunit alpha, CD51) is encoded by the ITGAV (also known as MSK8, VNRA, VTNR) gene (Gene ID: 3685) in human. Integrin alpha-V is a single-pass membrane protein that heterodimerizes with a beta subunit. The exact combination of various alpha and beta subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular region of the alpha subunit. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6, or beta-8 in a non-covalent manner. The structural and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrins bind to their ligands with low affinity and this binding occurs only when a certain minimum number of integrins are present at specific points known as focal contacts. The alpha-V is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate the mature form. The mature form contains an extracellular domain (aa 31-992), a transmembrane domain (aa 993-1016), and a cytoplasmic domain (aa 1017-1048). The alpha V is composed on a heavy chain (aa 31-889) and a light chain (aa 891-1048), which are linked by a disulfide bond. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Storage and Stability【储存及稳定性】
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 1
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