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Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

产品编号:4082521
规格:culture supernatant, clone 3L74, Upstate®
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4082521100 μL5520
产品别名

Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

ras homolog

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganisation, and membrane trafficking.
Specificity【特异性】
Broad species cross-reactivity expected based on sequence homology
Recognizes Rho (-A, -B, -C)
Immunogen【免疫原】
KLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility
Application【应用】
Research Sub Category
Cytoskeletal Signaling
Research Category
Signaling
This Anti-Rho (-A Antibody, -B, -C), clone 3L74 is validated for use in WB for the detection of Rho (-A, -B, -C).
Western Blot Analysis:
A 1:1000-1:4000 dilution of this lot detected Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. This antibody also recognized recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins.
Quality【质量】
routinely evaluated by immunoblot on modified RIPA lysates from HL-60 cells
Linkage【联系】
Replaces: 05-822
Physical form【外形】
Cultured supernantant in 0.05% sodium azide
Legal Information【法律信息】
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
culture supernatant
antibody product type
primary antibodies
clone【克隆】
3L74, monoclonal
species reactivity
human, rat
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NM_001664
UniProt accession no.【UniProt登记号】
P61586
shipped in【运输】
dry ice
Gene Information
human ... RHOA(387)
产品说明
Target description【目标描述】
Mr 22kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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