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Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27, rabbit monoclonal
产品编号: | 4082513 |
规格: | culture supernatant, clone MC27, Upstate® |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27, rabbit monoclonal
40S ribosomal protein S6, Phosphoprotein NP33, ribosomal protein S6
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that is known to be phosphorylated by both p70S6 Kinase as well as RSK as well as Ser240/244 which is thought to only be phosphorylated by p70S6K. |
Specificity【特异性】 | Broad species cross-reactivity predicted, due to sequence homology. Recognizes ribosomal protein S6 only when phosphorylated on Ser235. |
Immunogen【免疫原】 | Synthetic peptide containing the sequence …RRL[pS]SL… in which pS corresponds to phospho-serine at residue 235 of human ribosomal protein S6. The immunizing sequence is identical in mouse, rat, chicken and Xenopus. Epitope: Phosphorylated Ser235 |
Application【应用】 | Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27 is an antibody against phospho-Ribosomal Protein S6 (Ser235) for use in WB. Western Blot Analysis: A 1:500-1:2,000 dilution of this lot detected phosphorylated ribosomal protein S6 in lysates from serum stimulated 3T3 cells. Research Sub Category PI3K, Akt, & mTOR Signaling Research Category Signaling Apoptosis & Cancer |
Quality【质量】 | Routinely evaluated by western blot on serum stimulated 3T3 cell lysate. |
Physical form【外形】 | Cultured supernatant with 0.05% sodium azide. |
Analysis Note【分析说明】 | Control Serum stimulated 3T3 cell lysate. |
Legal Information【法律信息】 | UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | culture supernatant |
antibody product type | primary antibodies |
clone【克隆】 | MC27, monoclonal |
species reactivity | human, rat, mouse |
manufacturer/tradename | Upstate® |
technique(s) | western blot: suitable |
isotype【同位素/亚型】 | IgG |
NCBI accession no.【NCBI登记号】 | NM_001010.2 |
UniProt accession no.【UniProt登记号】 | P62753 |
shipped in【运输】 | dry ice |
Gene Information | human ... RPS6(6194) |
产品说明
Target description【目标描述】 | ~30 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |