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Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal

产品编号:4082502
规格:culture supernatant, clone NL314, Upstate®
包装规格:100 μl
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4082502100 μl6690
产品别名

Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal

H4K20me1, Histone H4 (mono methyl K20), histone 4, H4, histone H4, histone cluster 4, H4

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H4 on lys20 (H4-K20me3) selectively marks constitutive pericentromeric hetero-chromatin and imprinting control regions, while mono- and dimethylation are broadly distributed but principally enriched in euchromatin regions. Although the biological function of H4K20me remains poorly understood, studies suggest that H4-K20me2 is involved in the guidance of DNA repair proteins to DNA strand breaks, whereas H4K20me1 is associated with chromatin condensation processes. PR-Set7 (also known as SET8) specifically catalyzes monomethylation of H4K20me1.
Specificity【特异性】
Recognizes monomethyl-histone H4 (Lys20), Mr ~10kDa.
The immunizing sequence is conserved from Tetrahymena to human, so broad species cross-reactivity is expected.
Immunogen【免疫原】
KLH-conjugated, synthetic peptide containing the sequence …HR[mmK]V…, in which mmK corresponds to monomethyl-lysine at residue 20 of human histone H4.
Application【应用】
Use Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314 (rabbit monoclonal antibody) validated in WB, Mplex to detect monomethyl-Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20).
Immunoblot Analysis:
A 1:4000 dilution of a previous lot detected methylated histone H3 in acid extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3.

Multiplexing:
Beadlyte Histone-Peptide Specificity Assay: 1:2,000-1:30,000 dilutions of a previous lot were incubated with histone H4 peptides containing various modifications conjugated to Luminex microspheres. Specificity to monomethyl-histone H4 (Lys20) was demonstrated (Figure C).

Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Quality【质量】
Routinely evaluated by immunoblot analysis.
Immunoblot Analysis:
A 1:1000-1:2000 dilution of this lot detected monomethyl histone H4 (Lys20) in HeLa acid extracts. (Figure A).
Physical form【外形】
Cultured supernantant in 0.05% sodium azide
Analysis Note【分析说明】
Control
HeLa acid extracts
Legal Information【法律信息】
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
culture supernatant
antibody product type
primary antibodies
clone【克隆】
NL314, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
multiplexing: suitable
western blot: suitable
isotype【同位素/亚型】
IgG
NCBI accession no.【NCBI登记号】
NM_175054
UniProt accession no.【UniProt登记号】
P62805
shipped in【运输】
dry ice
产品说明
Target description【目标描述】
10kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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