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Anti-NeuN (rabbit) Antibody, Alexa Fluor® 488 Conjugate
产品别名
Anti-NeuN (rabbit) Antibody, Alexa Fluor® 488 Conjugate
NEUronal Nuclei, Neuronal Nuclei
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found. |
Specificity【特异性】 | This antibody recognizes NeuN at the N-terminus. The unconjugated rabbit Anti-NeuN (Catalog No. ABN78) has been shown to work in human. |
Immunogen【免疫原】 | GST-tagged recombinant protein corresponding to mouse NeuN. Epitope: N-terminus |
Application【应用】 | Immunocytochemistry analysis: A 1:100 dilution from a representative lot detected NeuN in rat E18 primary cortex cells. Detect NeuN (rabbit) using this Anti-NeuN (rabbit) Antibody, Alexa Fluor 488 Conjugate validated for use in IC, IH. Research Category Neuroscience Research Sub Category Developmental Neuroscience |
Quality【质量】 | Evaluated by Immunohistochemistry in adult mouse brain tissue. Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in adult mouse brain tissue. |
Physical form【外形】 | Affinity purified Purified rabbit polyclonal conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. |
Analysis Note【分析说明】 | Control Adult mouse brain tissue |
Legal Information【法律信息】 | ALEXA FLUOR is a registered trademark of Life Technologies |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
conjugate【偶联物】 | ALEXA FLUOR® 488 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | rat, mouse |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | The unconjugated parent antibody (Cat. No. ABN78) has an observed molecular weight at ~46/48 kDa An uncharacterized band appears at ~62 kDa in some lysates. |
Storage and Stability【储存及稳定性】 | Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |