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Milli-Mark® Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated

产品编号:4082397
规格:clone Bu-1, Milli-Mark®, from mouse
包装规格:100 TESTS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4082397100 TESTS4840
产品别名

Milli-Mark® Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated

brdu

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody′s special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.
Specificity【特异性】
Specifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.
Immunogen【免疫原】
Bromodeoxyuridine
Application【应用】
Research Sub Category
Cell Cycle, DNA Replication & Repair
Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.
Research Category
Epigenetics & Nuclear Function
Quality【质量】
Evaluated by flow cytometry with Jurkat Cells.
Physical form【外形】
Presentation needs to say: Purified mouse monoclonal IgG2a supernatant conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.
Protein G Purified
Analysis Note【分析说明】
Control
After incorporation of BrdU, all DNA containing species
Legal Information【法律信息】
ALEXA FLUOR is a registered trademark of Life Technologies
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
conjugate【偶联物】
ALEXA FLUOR® 488
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
Bu-1, monoclonal
species reactivity
human
species reactivity (predicted by homology)
all
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
isotype【同位素/亚型】
IgG2a
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
none given
Storage and Stability【储存及稳定性】
Stable for 6 months at 2-8°C from date of receipt. Protect from light.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
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