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Nova Taq DNA Polymerase
产品编号: | 4082204 |
规格: | Ultrapure recombinant enzyme for dependable PCR amplification |
包装规格: | 100 U,500 U,2500 U |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品性质
Quality Level【质量水平】 | 100 |
usage【用途】 | sufficient for 2000 reactions sufficient for 400 reactions sufficient for 80 reactions |
feature【特点】 | dNTPs included: no hotstart: no |
manufacturer/tradename | Novagen® |
storage condition【储存条件】 | OK to freeze |
technique(s) | PCR: suitable |
input | purified DNA |
storage temp.【储存温度】 | −20℃ |
基本信息
General description【一般描述】 | NovaTaq DNA Polymerase* is a premium quality, recombinant form of Thermus aquaticus DNA polymerase that is licensed for PCR. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest purity and reproducible perfomance, each preparation is extensively tested in a variety of quality control assays. NovaTaq DNA Polymerase has an integral 5′→3′ exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt, AccepTor, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl2, and 25 mM MgCl2 to enable convenient optimization of Mg2+ concentration. Source: Recombinant Thermus aquaticus DNA polymerase expressed in E. coli Concentration: 5.0 U/μl Purity: 95% homogeneous by SDS-PAGE Endonuclease: None detected Amplification effiency: Functional PCR Storage: –20℃ NovaTaq DNA polymerase is a recombinant form of Thermus aquaticus DNA polymerase. This enzyme is a non-proofreading DNA polymerase. NovaTaq DNA polymerase exhibits 5′-3′ DNA polymerase activity and lacks 3′- 5′ exonuclease activity. The preparation is >95% homogenous by sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE) and lacks detectable endonuclease activities. It is tested for several quality control assays. This enzyme produces PCR products with 3′-dA overhangs, suitable for cloning with Novagen®Perfectly Blunt®, AccepTor, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl2, and 25 mM MgCl2 to enable convenient optimization of Mg2+ concentration. Source: Recombinant Thermus aquaticus DNA polymerase expressed in E. coli Concentration: 5.0 U/μl Purity: 95% homogeneous by SDS-PAGE Endonuclease: None detected Amplification effiency: Functional PCR Storage: –20℃ | ||||||||||||
Application【应用】 | NovaTaq DNA polymerase has been used to check the success of the ligation step during cloning. It has also been used in the PCR amplification of cDNA. NovaTaq DNA Polymerase can be used in standard polymerase chain reaction (PCR) amplification protocols. It is also suitable for colony PCR for screening bacterial colonies. | ||||||||||||
Features and Benefits【特点和优势】 |
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Components【组分】 | • | 100 U, 500 U, or 5 × 500 U | NovaTaq DNA Polymerase | • | 1 or 2 or 7 × 1.5 ml | 10X NovaTaq Buffer with MgCl₂ | • | 1 or 2 or 7 × 1.5 ml | 10X NovaTaq Buffer without MgCl₂ | • | 1 or 2 or 7 × 1.5 ml | 25 mM MgCl₂ | |
Warning【警告】 | Toxicity: Multiple Toxicity Values, refer to MSDS (O) | ||||||||||||
Unit Definition【单位定义】 | One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 74°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-32P]dCTP, and activated salmon sperm DNA. | ||||||||||||
Legal Information【法律信息】 | Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA. NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 2 |