您的位置:首页 > 产品中心 > ColiComplete
ColiComplete
产品编号: | 4082196 |
规格: | BioControl, For detection Total Coliforms and E. coli in food products |
包装规格: | 100 TESTS,3000 TESTS |
产品类别: | 进口试剂 |
品牌: | Millipore |
优惠价: | 立即咨询 |
产品性质
material【物料】 | paper disks (containing 5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside (X-Gal) and 4-methyl umbelliferyl-ß-D-glucuronide (MUG)) |
agency | AOAC® 992.30 (OMA) |
shelf life【保质期】 | 6‑18 mo. |
packaging【包装】 | pkg of 100 pkg of 3000 |
manufacturer/tradename | BioControl Systems 10800-100 BioControl Systems 10800-3K |
application(s) | food and beverages pathogen testing |
storage temp.【储存温度】 | 2-8℃ |
suitability【适用性】 | coliforms: Escherichia coli |
基本信息
General description【一般描述】 | ColiComplete is a patented substrate supporting disc method containing both XGAL and MUG. It is designed to simultaneously detect Total Coliforms and E. coli in food products. The β-galactosidase enzyme produced by coliforms reacts with the chromogenic XGAL substrate to produce a visually detectable insoluble blue dye. The β-glucuronidase enzyme found in E. coli reacts with the MUG substrate to produce a blue fluorescent reaction under long wave UV light. |
Application【应用】 | AOAC Official Method 992.30 |
Features and Benefits【特点和优势】 |
|
Components【组分】 | Bottle of reagent discs |
Principle【原理】 | ColiComplete contains 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal) and 4-methyl umbelliferyl-β-D-glucuronide (MUG). Discs are added to LST inoculated with selected dilutions of samples. Samples are incubated at 35 - 37 oC and examined after 24 and 48 ± 2 h for confirmed total coliforms and after 30 ± 2 h for confirmed E. coli results. β-Galactosidase, from coliforms present in samples, cleaves X-Gal into 5-bromo-4-chloro-indoxyl intermediate which undergoes oxidation to yield water-insoluble blue dimer, visually detectable on disc or in surrounding medium as confirmed positive result for total coliform activity. β-Glucuronidase, from E. coli present in samples, cleaves MUG into glucuronide and methyl umbelliferone, which fluoresces under long wave UV light (366 nm) as confirmed positive result for E. coli presence. NOTE: As E. coli O157:H7 does not produce β-glucuronidase, ColiComplete is not suitable for the detection of E. coli O157:H7. |
Testing Method【测试方法】 | X-Gal, MUG |
Legal Information【法律信息】 | AOAC is a registered trademark of AOAC International |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 3 |