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ColiComplete

产品编号:4082196
规格:BioControl, For detection Total Coliforms and E. coli in food products
包装规格:100 TESTS,3000 TESTS
产品类别:进口试剂
品牌:Millipore
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4082196100 TESTS1132
40821963000 TESTS22920
产品性质
material【物料】
paper disks (containing 5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside (X-Gal) and 4-methyl umbelliferyl-ß-D-glucuronide (MUG))
agency
AOAC® 992.30 (OMA)
shelf life【保质期】
6‑18 mo.
packaging【包装】
pkg of 100
pkg of 3000
manufacturer/tradename
BioControl Systems 10800-100
BioControl Systems 10800-3K
application(s)
food and beverages
pathogen testing
storage temp.【储存温度】
2-8℃
suitability【适用性】
coliforms: Escherichia coli
基本信息
General description【一般描述】
ColiComplete is a patented substrate supporting disc method containing both XGAL and MUG. It is designed to simultaneously detect Total Coliforms and E. coli in food products. The β-galactosidase enzyme produced by coliforms reacts with the chromogenic XGAL substrate to produce a visually detectable insoluble blue dye. The β-glucuronidase enzyme found in E. coli reacts with the MUG substrate to produce a blue fluorescent reaction under long wave UV light.
Application【应用】
AOAC Official Method 992.30
Features and Benefits【特点和优势】
  • ColiComplete provides confirmed quantitative E. coli results in as few as 28 hours and confirmed total Coliforms result in 48 hours.
  • Just add the patented ColiComplete disc to Lauryl Sulfate Broth (LST) tubes at the time of inoculation, incubate and read results. Use the Most Probably Number (MPN) format for quantitative results. No transfers are necessary.
  • Simultaneous detection of both E. coli and total Coliforms from a single test. Results are confirmed and require no subculturing.

Components【组分】
Bottle of reagent discs
Principle【原理】
ColiComplete contains 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal) and 4-methyl umbelliferyl-β-D-glucuronide (MUG). Discs are added to LST inoculated with selected dilutions of samples. Samples are incubated at 35 - 37 oC and examined after 24 and 48 ± 2 h for confirmed total coliforms and after 30 ± 2 h for confirmed E. coli results. β-Galactosidase, from coliforms present in samples, cleaves X-Gal into 5-bromo-4-chloro-indoxyl intermediate which undergoes oxidation to yield water-insoluble blue dimer, visually detectable on disc or in surrounding medium as confirmed positive result for total coliform activity. β-Glucuronidase, from E. coli present in samples, cleaves MUG into glucuronide and methyl umbelliferone, which fluoresces under long wave UV light (366 nm) as confirmed positive result for E. coli presence.
NOTE: As E. coli O157:H7 does not produce β-glucuronidase, ColiComplete is not suitable for the detection of E. coli O157:H7.
Testing Method【测试方法】
X-Gal, MUG
Legal Information【法律信息】
AOAC is a registered trademark of AOAC International
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
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