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Magna ChIRP U2snRNA Probe

产品编号:4082162
规格:The Magna ChIRP U2snRNA contains a 20-mer DNA oligonucleotide complementary to the sequence of Human RNA, U2 small nuclear 1.
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4082162The Magna ChIRP U2snRNA contains a 20-mer DNA oligonucleotide complementary to the sequence of Human RNA, U2 small nuclear 1.2680
基本信息
eCl@ss
32161000
General description【一般描述】
Pervasive genomic transcription and the role of non-coding RNAs in this process is a rapidly evolving field that remains to be thoroughly explored in gene regulation.
Historically, chromatin immunoprecipitation (ChIP) has been used to interrogate association of proteins with genomic DNA sequences. Given that chromatin contains not only DNA and DNA-binding proteins, but also RNA-binding proteins and RNA, new methods have been developed to map the association of these RNA molecules to distinct regions of the genome. One such method, designated ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target. This unique approach allows isolation of chromatin-associated RNAs (e.g. lncRNAs) within specific regions of genomic DNA sequences, as well as proteomic analysis of protein components of the lncRNA regulatory complexes. The Magna ChIRP® RNA Interactome Kits (cat. # 17-10494 and 17-10495) facilitate the use of this method and has been proven compatible with this probe set.
The Magna ChIRP U2snRNA contains a 20-mer DNA oligonucleotide complementary to the sequence of Human RNA, U2 small nuclear 1. The probe is biotin-TEG-labeled at the 3-prime end. The oligonucleotide is supplied ready for use in the ChIRP method.
Application【应用】
The Magna ChIRP U2snRNA contains a 20-mer DNA oligonucleotide complementary to the sequence of Human RNA, U2 small nuclear 1.
Quality【质量】
Verified by Electrospray Ionization Mass Spectrometry. Final yields are determined using UV absorbance at OD260. Concentrations are determined using UV absorbance at OD260 before pooling. Oligos will manufactured under ISO 9001 conditions.
Physical form【外形】
10 mM Tris-HCl, 1mM EDTA, pH 8.0
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
Legal Information【法律信息】
MAGNA CHIRP is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
manufacturer/tradename
Magna ChIRP®
technique(s)
DNA sequencing: suitable
RNA extraction: suitable (nuclear function kit)
RNA purification: suitable (with magnetic beads)
activity assay: suitable (protein interaction)
nucleic acid detection: suitable (RNA)
NCBI accession no.【NCBI登记号】
NR_002716
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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