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Trypsin, Bovine Pancreas

产品编号:4081399
CAS NO:9002-07-7
包装规格:25 MU
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
408139925 MU7420
产品别名

9002-07-7

Trypsin, Bovine Pancreas

基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
3.4.21.4 ( BRENDA | IUBMB )
General description【一般描述】
Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Has an optimal pH of 8.5-8.8.
Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units.
Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides and proteins at the C-terminal side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Note: 1 MU = 1,000,000 units.
Warning【警告】
Toxicity: Irritant (B)
Unit Definition【单位定义】
One USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
Physical form【外形】
Salt-free
Reconstitution【重悬】
Following reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.
Other Notes【其他说明】
Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.
Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem.239, 1799.
Hummel, B.C.W. 1955. Can. J. Biochem. Physiol.37, 1393.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
form【形式】
solid
Specific Activity
≥2,500 USP units/mg dry wt
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
desiccated (hygroscopic)
solubility【溶解性】
HCl: 1 mM
neutral buffer: soluble
water: soluble
foreign activity【异质活性】
Chymotrypsin ≤50 NF units/mg dry wt.
shipped in【运输】
ambient
storage temp.【储存温度】
−20℃
packaging【包装】
25 mu in Plastic ampoule
2.5 mu in Glass bottle
安全信息
Pictograms【象形图】
GHS07,GHS08
Signal word【警示用语:】
Danger
Hazard Statements
H315 - H319 - H334 - H335
Precautionary Statements
P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
Hazard Classifications【危险分类】
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs【靶器官】
Respiratory system
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 1
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