Anti-Human IgG (Fc Specific)-Agarose antibody produced in goat

MFCD02183598

NA.46

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is specific for the Fc fragment of human IgG when tested against purified human IgA, IgG, IgM, Fc, and kappa and lambda light chains. No reactivity with mouse or rat IgG is seen by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling.

Fc fragment of human IgG.

Goat Anti-Human IgG (Fc Specific)-Agarose antibody is suitable for use in Ouchterlony double diffusion. The antibody has also been used for affinity purification assays.
Anti-Human IgG (Fc Specific)-Agarose antibody produced in goat has also been used to purify galanin 1 (Gala1), 3Gal-substituted P-selectin glycoprotein ligand-1 (PSGL-1)/hIgG1 from supernatants of transfected COS cells.
Immunoprecipitation assays were performed using goat anti-human IgG (Fc specific) crosslinked to agarose beads. The beads were diluted in 1 ml of 5% FBS/DMEM for 2 hours at 4 degrees.

Antibody adsorbed with mouse and rat IgG

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

200

goat

agarose conjugate

affinity isolated antibody

secondary antibodies

polyclonal

PBS suspension

human

rat, mouse

Ouchterlony double diffusion: suitable

2 mg/mL, resin binding capacity (human IgG)

2-8℃

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10 - Combustible liquids

WGK 3