单克隆抗磷酸丝氨酸

NA.44

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediate numerous signalling pathways in both prokaryotic and Eukaryotic cells. Cellular proteins with phosphorylated serine increase many fold by the activation of serine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.
Monoclonal Anti-Phosphoserine may be used for the identification of proteins containing phosphorylated serine both as the free amino acid or when conjugated to carriers such as BSA or KLH. The purified mouse immunoglobulin from ascites fluid is conjugated to biotinamidohexanoic acid N-hydroxysuccinimide ester. This covalent coupling of biotin to the immunoglobulin allows for the binding of avidin or streptavidin bearing a variety of different labels.
Monoclonal Anti-Phosphoserine (mouse IgG1 isotype) is derived from the PSR-45 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice.

通过 ELISA 和斑点印迹，该抗体以游离氨基酸形式或与载体(如 BSA 或 KLH)的缀合，与磷酸化丝氨酸特异性反应。与非磷酸化丝氨酸、磷酸苏氨酸、磷酸酪氨酸、AMP 或 ATP 无交叉反应。该抗体已用在免疫印迹中定位一些含磷酸丝氨酸蛋白质。由于识别位点的空间位阻，该抗体可能无法识别已知含有磷酸化丝氨酸的某些蛋白质。

磷酸丝氨酸与钉形贝血蓝蛋白(KLH)结合。

Monoclonal Anti-Phosphoserine-Biotin antibody produced in mouse has been used in western blotting.
Monoclonal Anti-Phosphoserine−Biotin antibody may be used in indirect ELISA at a recommended dilution of 1:50,000 - 1:100,000. The antibody may be used for detection by dot blot at a working dilution of 1:200,000.

Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

200

mouse

biotin conjugate

purified immunoglobulin

primary antibodies

PSR-45, monoclonal

buffered aqueous solution

direct ELISA: 1:50,000-1:100,000 using phosphoserine-BSA at 10 μg/ml, and ExtrAvidin-HRP at 2 μg/ml
dot blot: 1:200,000 using 40 ng phosphoserine-BSA/dot and ExtrAvidin-HRP at 1 μg/ml

IgG1

dry ice

−20℃

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12 - Non Combustible Liquids

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