单克隆抗肌动蛋白抗体

NA.41

该抗体可识别在所有肌动蛋白亚型中均保守的C末端表位。它能够特异性标记各种组织和物种中的肌动蛋白。 被抗体识别的表位能够耐受甲醛固定和石蜡包埋。锌-福尔马林、B5、乙醇、methacarn、Brunnel's或Bouin's溶液也可用作固定液。
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

与多抗原肽(MAP)骨架连接的合成肌动蛋白C末端肽

肌动蛋白单克隆抗体被用于Cos-7细胞裂解物的蛋白质免疫印迹分析，以确保等量蛋白质上样。
已使用肌动蛋白单克隆抗体作为一抗，对MDCK细胞裂解物进行蛋白质免疫印迹分析。
Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts.

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

200

mouse

FITC conjugate

purified immunoglobulin

primary antibodies

AC-40, monoclonal

buffered aqueous solution

antigen 42 kDa

human, carp, Xenopus, canine, sheep, pig, mouse, rabbit, rat, hamster, chicken, snail, bovine, viper, guinea pig, goat

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

IgG2a

dry ice

−20℃

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12 - Non Combustible Liquids

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