PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb

rabbit

200

affinity isolated antibody

primary antibodies

polyclonal

liquid

≤0.1% sodium azide as preservative

human

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−20℃

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser³⁰⁰ in HEK293 cells.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser³⁰⁰.
This PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α
Human

Immunoblotting (0.5 μg/ml)
Immunocytochemistry (1 μg/ml)

Toxicity: Standard Handling (A)

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.

Following initial thaw, aliquot and freeze (-20°C).

Negative Control
HEK293 cells treated with dichloroacetate
Positive Control
HEK293 cells

Due to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.
Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

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