PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb

rabbit

200

affinity isolated antibody

primary antibodies

polyclonal

liquid

≤0.1% sodium azide as preservative

human, mouse, rat

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−20℃

50 μg in Plastic ampoule

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser²³².
This PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb is validated for use in Immunoblotting, IF, IP for the detection of PDH-E1α (pSer²³²).
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser²³² in HEK293 cells.

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α
Human

Immunoblotting (0.25 g/ml)
Immunofluorescence (Please see comments)
Immunoprecipitation (1 g/ml)

Toxicity: Standard Handling (A)

In PBS.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
HEK293 cells

This antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

12 - Non Combustible Liquids

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