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Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–FITC antibody produced in sheep

产品编号:4077502
规格:affinity isolated antibody, buffered aqueous solution
包装规格:0.5 ML,1 ML,2 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40775020.5 ML2070
40775021 ML3580
40775022 ML6170
基本信息
MDL number
MFCD00162642
NACRES
NA.46
General description【一般描述】
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody is specific for ) F(ab′)2 fragment of mouse IgG1, G2a, G2b, and G3 subclasses. The antibody preparation is solid phase adsorbed with human serum proteins prior to conjugation to minimize cross reactivity. The antibody preparation is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment is specific for mouse IgG subclasses G1, G2a, G2b, and G3 as demonstrated by Ouchterlony double diffusion using mouse myeloma proteins. The antibody product can be used to avoid background staining due to the presence of Fc receptors.
Specificity【特异性】
Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Immunogen【免疫原】
Pepsin digest of antiserum
Mouse IgG
Application【应用】
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody may be used in direct immunofluorescence at a minimum antibody dilution of 1:128. The antibody was used at a working dilution of 1:25 to stain microtubules in epidermal cells of Vigna unguiculata or cowpea It was also used for flow cytometry analysis using COS cells and human peripheral blood mononuclear cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Other Notes【其他说明】
Antibody adsorbed with human serum proteins.
Physical form【外形】
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note【制备说明】
Adsorbed to reduce background staining with human samples.
产品性质
Quality Level【质量水平】
300
biological source【生物来源】
sheep
conjugate【偶联物】
FITC conjugate
antibody form【抗体形式】
affinity isolated antibody
antibody product type
secondary antibodies
clone【克隆】
polyclonal
form【形式】
buffered aqueous solution
technique(s)
direct immunofluorescence: 1:128
storage temp.【储存温度】
2-8℃
packaging【包装】
0.5 mL in glass insert
1, 2 mL in glass bottle
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
nwg
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