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VCaP Cell Line human

产品编号:4077158
规格:06020201, Prostate Cancer Metastasis
包装规格:20μl,5μg,1VL
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
407715820μl4470
40771585μg4630
40771585μg5400
40771581VL11270
产品性质
biological source【生物来源】
human prostate (metastasis)
growth mode【生长模式】
Adherent
karyotype【核型】
Refer to reference (Korenchuk et al., 2001)
morphology【形态学】
Epithelial
products【产品】
Not specified
receptors【受体】
Androgen receptor
technique(s)
cell culture | mammalian: suitable
relevant disease(s)【相关疾病】
metastasis
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
基本信息
Cell Line Origin【细胞系来源】
Human Prostate Cancer Metastasis
Cell Line Description【细胞系描述】
This Vertebral-Cancer of the Prostate (VCaP) cell line was established from prostate cancer tissue harvested from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. The cells are reported to express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitive in vitro and in vivo. This cell line offers a cell-based model system of human prostate cancer. The cells are tumourigenic in SCID mice.
DNA Profile【DNA图谱分析】
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19
Culture Medium【培养基】
DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine【传代培养常规】
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37℃. Pellet cells by centrifugation and remove trypsin after inactivation of trypsin at each subculture.
Other Notes【其他说明】
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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