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Propidium Iodide-CAS 25535-16-4-Calbiochem

产品编号:4077103
规格:Membrane impermeable DNA intercalator.
CAS NO:25535-16-4
包装规格:50 MG,100 MG,250 MG,1 G
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
407710350 MG1590
4077103100 MG3230
4077103250 MG6800
40771031 G21470
产品别名

25535-16-4

Propidium Iodide-CAS 25535-16-4-Calbiochem

基本信息
Empirical Formula【经验(实验)分子式】
C27H34I2N4
Molecular weight
668.39
MDL number
MFCD00011921
General description【一般描述】
Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M-1cm-1 (493 nm); 55,700 M-1cm-1 (287 nm); 13,800 M-1cm-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.
Biochem/physiol Actions【生化/生理作用】
Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no
Cell permeable: no
Warning【警告】
Toxicity: Irritant (B)
Preparation Note【制备说明】
Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.
Reconstitution【重悬】
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes【其他说明】
Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
form【形式】
solid
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
protect from light
color【颜色】
dark red
input
sample type intact cells
fluorescence【荧光】
λex ~536 nm
λem ~617 nm
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
packaging【包装】
1 g in Plastic ampoule
250 mg in Plastic ampoule
50, 100 mg in Glass bottle
安全信息
Pictograms【象形图】
GHS08
Signal word【警示用语:】
Warning
Hazard Statements
H341
Precautionary Statements
P201 - P202 - P280 - P308 + P313 - P405 - P501
Hazard Classifications【危险分类】
Muta. 2
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 3
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