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PNT2 Cell Line human

产品编号:4077083
规格:95012613, Normal prostate epithelium immortalized with SV40.
包装规格:5μg,1 VIAL
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40770835μg4630
40770831 VIAL11270
产品性质
biological source【生物来源】
human prostate
growth mode【生长模式】
Adherent
karyotype【核型】
Not specified
morphology【形态学】
Epithelial
products【产品】
Not specified
receptors【受体】
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
基本信息
Cell Line Origin【细胞系来源】
Human prostate normal, immortalised with SV40
Cell Line Description【细胞系描述】
Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice.
Application【应用】
Biology of prostate cells, model of differentiated prostate epithelial cells.
PNT2 cell line has been used:
  • to assess the effects of Verapamil, an L-type calcium channel blocker on autophagy
  • to assess the cytotoxicity of aqueous dilutions of a universal self-priming dental adhesive (DA)
  • to study the expression of soluble adenylyl cyclase (sAC) in the cells
DNA Profile【DNA图谱分析】
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,13
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 6,9.3
TPOX: 8,9
vWA: 17
Culture Medium【培养基】
RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).
Subculture Routine【传代培养常规】
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
Other Notes【其他说明】
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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