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PNT2 Cell Line human
产品编号: | 4077083 |
规格: | 95012613, Normal prostate epithelium immortalized with SV40. |
包装规格: | 5μg,1 VIAL |
产品类别: | 进口试剂 |
优惠价: | 立即咨询 |
产品性质
biological source【生物来源】 | human prostate |
growth mode【生长模式】 | Adherent |
karyotype【核型】 | Not specified |
morphology【形态学】 | Epithelial |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Human prostate normal, immortalised with SV40 |
Cell Line Description【细胞系描述】 | Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice. |
Application【应用】 | Biology of prostate cells, model of differentiated prostate epithelial cells. PNT2 cell line has been used:
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DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X,Y CSF1PO: 11,13 D13S317: 8,13 D16S539: 9 D5S818: 12,13 D7S820: 11 THO1: 6,9.3 TPOX: 8,9 vWA: 17 |
Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |