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胰蛋白酶原 来源于牛胰腺
Trypsinogen from bovine pancreas
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产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
4076656 | 250 MG | 2610 | |||
4076656 | 1 G | 7840 |
产品别名
9002-08-8
胰蛋白酶原 来源于牛胰腺
Trypsinogen from bovine pancreas
基本信息
EC number【EC 号】 | 232-651-3 |
NACRES | NA.56 |
General description【一般描述】 | Trypsinogen is a proenzyme (zymogen) that is activated to form trypsin. It is synthesized in the pancreas and activated by enterokinase once it reaches the lumen of the small intestine. Bovine trypsinogen is a single polypeptide chain of 229 amino acids that is cross linked by six disulfide bridges. Enterokinase cleaves a hexapeptide to from the NH2 terminus of trypsinogen at the Lys6 - Ile7 peptide bond and activates it. Trypsin, thus formed, autocatalytically activates more trypsinogen to trypsin. This native form of trypsin is called β-trypsin, which undergoes autolysis at Lys131 - Ser132 resulting in α-trypsin that is held together by disulfide bridges. Trypsin is a serine protease with His46 and Ser183 at the active site. The pH optimum of trypsin is 7 - 9. |
Application【应用】 | Trypsinogen from bovine pancreas is suitable for use in:
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Biochem/physiol Actions【生化/生理作用】 | Phytic acid complexed with calcium has been shown to increase the secretion of trypsinogen unable to be cleaved for activation. It also reduced the stabilization effect of calcium on activated trypsin. The active form of trypsinogen, referred to as trypsin, cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. It also hydrolyzes ester and amide linkages of synthetic derivatives of amino acids such as, benzoyl L-arginine ethyl ester (BAEE), p-toluenesulfonyl-L-arginine methyl ester (TAME), etc. |
Unit Definition【单位定义】 | One BAEE unit is equal to a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 ℃ and a reaction volume of 3.2 mL (1 cm light path). |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | bovine pancreas |
Assay【测定】 | 85-100% (UV) |
form【形式】 | essentially salt-free, lyophilized powder |
Specific Activity | ≥10,000 BAEE units/mg protein (E |
mol wt【分子量】 | 23,981 Da by calculation |
technique(s) | mass spectrometry (MS): suitable |
solubility【溶解性】 | H2O: soluble 10 mg/mL |
UniProt accession no.【UniProt登记号】 | Q29463 |
storage temp.【储存温度】 | −20℃ |
Gene Information | bovine ... TRYP8(282603) |
packaging【包装】 | 1 g in glass bottle 250 mg in glass bottle |
安全信息
Pictograms【象形图】 | GHS08 |
Signal word【警示用语:】 | Danger |
Hazard Statements | H315 - H319 - H334 |
Precautionary Statements | P302 + P352 - P305 + P351 + P338 |
Hazard Classifications【危险分类】 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 |
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Faceshields, Gloves, type N95 (US) |