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胰蛋白酶原 来源于牛胰腺

Trypsinogen from bovine pancreas
产品编号:4076656
规格:essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin)
CAS NO:9002-08-8
包装规格:250 MG,1 G
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
4076656250 MG2610
40766561 G7840
产品别名

9002-08-8

胰蛋白酶原 来源于牛胰腺

Trypsinogen from bovine pancreas

基本信息
EC number【EC 号】
232-651-3
NACRES
NA.56
General description【一般描述】
Trypsinogen is a proenzyme (zymogen) that is activated to form trypsin. It is synthesized in the pancreas and activated by enterokinase once it reaches the lumen of the small intestine. Bovine trypsinogen is a single polypeptide chain of 229 amino acids that is cross linked by six disulfide bridges. Enterokinase cleaves a hexapeptide to from the NH2 terminus of trypsinogen at the Lys6 - Ile7 peptide bond and activates it. Trypsin, thus formed, autocatalytically activates more trypsinogen to trypsin. This native form of trypsin is called β-trypsin, which undergoes autolysis at Lys131 - Ser132 resulting in α-trypsin that is held together by disulfide bridges. Trypsin is a serine protease with His46 and Ser183 at the active site. The pH optimum of trypsin is 7 - 9.
Application【应用】
Trypsinogen from bovine pancreas is suitable for use in:
  • the secondary structure analysis of proteins in H2O solution using single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopy
  • tuning and calibration of electrospray ionization quadrupole time-of-flight (ESI-Q-TOF) mass spectrometer
  • the secondary structure analysis of proteins by infrared (IR) spectroscopy
  • SDS-PAGE as a molecular weight standard (24kDa)
Biochem/physiol Actions【生化/生理作用】
Phytic acid complexed with calcium has been shown to increase the secretion of trypsinogen unable to be cleaved for activation. It also reduced the stabilization effect of calcium on activated trypsin. The active form of trypsinogen, referred to as trypsin, cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. It also hydrolyzes ester and amide linkages of synthetic derivatives of amino acids such as, benzoyl L-arginine ethyl ester (BAEE), p-toluenesulfonyl-L-arginine methyl ester (TAME), etc.
Unit Definition【单位定义】
One BAEE unit is equal to a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 ℃ and a reaction volume of 3.2 mL (1 cm light path).
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
bovine pancreas
Assay【测定】
85-100% (UV)
form【形式】
essentially salt-free, lyophilized powder
Specific Activity
≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin)
mol wt【分子量】
23,981 Da by calculation
technique(s)
mass spectrometry (MS): suitable
solubility【溶解性】
H2O: soluble 10 mg/mL
UniProt accession no.【UniProt登记号】
Q29463
storage temp.【储存温度】
−20℃
Gene Information
bovine ... TRYP8(282603)
packaging【包装】
1 g in glass bottle
250 mg in glass bottle
安全信息
Pictograms【象形图】
GHS08
Signal word【警示用语:】
Danger
Hazard Statements
H315 - H319 - H334
Precautionary Statements
P302 + P352 - P305 + P351 + P338
Hazard Classifications【危险分类】
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 1
Personal Protective Equipment【个人防护装备】
Eyeshields, Faceshields, Gloves, type N95 (US)
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