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ExtrAvidin®-Agarose, high binding

产品编号:4076370
规格:For purification of biotinylated macromolecules and complexes
包装规格:1 ML,5 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40763701 ML2620
40763705 ML7960
产品性质
form【形式】
aqueous suspension
suspension
feature【特点】
For purification of biotinylated macromolecules and complexes
concentration【浓度】
50%
matrix【基质】
4% agarose (beaded)
matrix activation【基质活化】
cyanogen bromide
storage temp.【储存温度】
2-8℃
基本信息
General description【一般描述】
ExtrAvidin® is a unique de-glycosylated Avidin modified form of an affinity purified egg white avidin. The ExtrAvidin is conjugated to cyanogen bromide-activated agarose beads.
ExtrAvidin® is a tetrameric protein containing four biotin binding sites. The Avidin-Biotin high affinity interaction (Kd=10-15M) is considered one of the strongest non-covalent interactions known in nature. The use of Avidin-Biotin complex for affinity purification was described in the first half of 1970 and since then it was successfully utilized in numerous studies and biotechnological applications.1-3 This was achieved due to the ability to chemically coupe the small Biotin molecule with different binders, without disturbing its function or structure, thus allowing unique interaction with a variety of Avidin carriers including: protein or DNA molecules, Avidin protein bound to a solid surface matrix, reporter molecules, probes or carriers,. Among the known Avidin-Biotin interaction based applications are purification, enrichment, detection, amplification and other research medical and industrial processes. In addition, the ExtrAvidin® high specificity binding to Biotin, together with the low background staining, grant it significant advantage compared to the non-modified Avidin or Streptavidin produced by Streptomyces avidinii.
Application【应用】
ExtrAvidin®-Agarose provides high affinity with high specificity binding to Biotin (also known as vitamin B7) including biotinylated proteins and biotin-tagged fusion proteins. The product may be used in various immunological techniques, including Immunoprecipitation and Immunoaffinity purification.
Caution【注意】
DO NOT FREEZE
Physical form【外形】
ExtrAvidin®-Agarose is provided as suspension at a 1:1 ratio, in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Legal Information【法律信息】
ExtrAvidin is a registered trademark of sigma-aldrich Co. LLC
产品说明
Disclaimer【免责声明】
This product is for research use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
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