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Bisbenzimide H 33258 Fluorochrome, Trihydrochloride-CAS 23491-45-4-Calbiochem

产品编号:4075580
规格:Membrane-permeable, adenine-thymine-specific fluorescent stain.
CAS NO:23491-45-4
包装规格:100 MG,250 MG,1 G
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
4075580100 MG1800
4075580250 MG4030
40755801 G11400
产品别名

23491-45-4

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride-CAS 23491-45-4-Calbiochem

2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258

基本信息
Empirical Formula【经验(实验)分子式】
C25H24N6O · 3HCl
Molecular weight
533.88
MDL number
MFCD00012679
General description【一般描述】
Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.
Membrane-permeant, adenine-thymidine specific fluorescent stain (excitation maximum: 346 nm; emission maximum: 460 nm). Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.
Biochem/physiol Actions【生化/生理作用】
Cell permeable: yes
Product does not compete with ATP.
Reversible: no
Primary Target
Adenine-thymine-specific fluorescent stain
Warning【警告】
Toxicity: Carcinogenic / Teratogenic (D)
Preparation Note【制备说明】
Precipitates in phosphate buffered solutions
Reconstitution【重悬】
Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.
Other Notes【其他说明】
Werstuck, G., and Green, M.R. 1998. Science 282, 296.
Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
Tarshis, M., et al. 1994. J. Immunol. Methods168, 245.
Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods162, 41.
Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA85, 8939.
Cesarone, C.F., et al. 1979. Anal. Biochem.100, 188.
Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem.24, 24.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
Assay【测定】
≥98% (HPLC)
form【形式】
solid
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
protect from light
color【颜色】
yellow-green
fluorescence【荧光】
λex 346  nm
λem 460 nm
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
InChI
1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,26,28H,10-13H2,1H3,(H,27,29);3*1H
InChI key
OKQAASMPIOSOEX-UHFFFAOYSA-N
packaging【包装】
100, 250 mg in Plastic ampoule
1 g in Plastic ampoule
安全信息
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 3
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