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HeLa9903

产品编号:4075346
规格:from human cervix, 11033105
包装规格:1 VIAL
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40753461 VIAL11270
产品别名

HeLa9903

HeLa

产品性质
biological source【生物来源】
human cervix
growth mode【生长模式】
Adherent
karyotype【核型】
Not specified
morphology【形态学】
Epithelial
products【产品】
Not specified
receptors【受体】
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)【相关疾病】
cancer
shipped in【运输】
dry ice
基本信息
Cell Line Origin【细胞系来源】
HeLa, genetic modification, the hER alpha expression construct, Oestrogen-Responsive Element
Cell Line Description【细胞系描述】
HeLa9903 is a HeLa cell line stably transfected with two constructs: the hER alpha expression construct; a firefly luciferase reporter construct bearing Oestrogen-Responsive Element (ERE) driven by a mouse metallothionein (MT) promoter TATA element. HeLa9903 can be used to test for evaluation of chemicals based on OECD Test Guideline: The stably transformed human oestrogen receptor-alpha transcriptional; activation assay for detection of oestrogenic activity of chemicals.
DNA Profile【DNA图谱分析】
STR-PCR Data:
Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Culture Medium【培养基】
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 16μg/ml Blasticidin S HCL + 800μg/ml G418.
Subculture Routine【传代培养常规】
Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 ℃.
Other Notes【其他说明】
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Legal Information【法律信息】
US7537904B1 N Ooe & Matsunaga H 2009.Cell for measuring the ability to control the activity of a ligand-responsive transcription control factor.
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