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Cas9 Hygromycin Lenti Plasmid

产品编号:4074269
包装规格:1 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40742691 μG2730
产品性质
recombinant【重组】
expressed in E. coli
packaging【包装】
vial of 50 μL
concentration【浓度】
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)
CRISPR
selection
blasticidin
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
基本信息
General description【一般描述】
This product is a lentiviral plasmid that utilizes the EF1 alpha promoter to drive expression of Cas9 and a hygromycin resistance cassette linked by a 2A peptide (EF1a-Cas9-2A-Hygro) allowing for easy visualization of successful transfection or transduction. Use Sigma′s lentiviral Cas9 plasmid for generation of lentiviral particles and efficient production of stable cell lines expressing Cas9 for CRISPR based genome editing. Sigma′s Cas9-Hygro lenti plasmid is one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors.

To order gRNA in any format click here
Application【应用】
Functional Genomics/Target Validation
  • Creation of gene knockouts in multiple cell lines
  • Complete knockout of genes not amenable to RNAi
  • Manufacture of Cas9 expressing Lentiviral Particles
Features and Benefits【特点和优势】
  • Highly specific and highly active
  • Sequence verified
  • Ready to use purified plasmid DNA
Principle【原理】
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Legal Information【法律信息】
CRISPR Label License

Lentiviral, WPRE, and Evrogen Licenses
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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