MISSION^® pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer

NA.51

The MISSION pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.
High titer virus is useful when performing in vivo research.
When conducting experiments using MISSION^® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

To see more application data, protocols, vector maps visit sigma.com/shrna.

MISSION is a registered trademark of sigma-aldrich Co. LLC

200

MISSION^®

≥1x10⁹ VP/ml (via p24 assay)

dry ice

−70℃

12 - Non Combustible Liquids

WGK 3