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Lenti CRISPR Universal Non-Target Control#1 Transduction Particles (LV04 vector)
基本信息
NACRES | NA.51 |
General description【一般描述】 | The Lenti CRISPR Non-Target Control#1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Contro#1 usesl a dual-component system consisting of U6-driven guide RNA (non-target#1) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector. Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids. |
Application【应用】 | 功能性基因组学研究/筛选/靶标验证 |
Other Notes【其他说明】 | Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. |
Legal Information【法律信息】 | CRISPR Use License Agreement Lentiviral, WPRE, and Evrogen Licenses |
产品性质
Quality Level【质量水平】 | 200 |
packaging【包装】 | vial of 200 μL |
concentration【浓度】 | (1x106 TU/ml (via p24 titering assay)) |
application(s) | CRISPR |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |