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Lenti CRISPR Universal Non-Target Control#1 Transduction Particles (LV04 vector)

产品编号:4074061
包装规格:1 EA
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40740611 EA3680
基本信息
NACRES
NA.51
General description【一般描述】
The Lenti CRISPR Non-Target Control#1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Contro#1 usesl a dual-component system consisting of U6-driven guide RNA (non-target#1) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
Application【应用】
功能性基因组学研究/筛选/靶标验证
Other Notes【其他说明】
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.
Legal Information【法律信息】
CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses
产品性质
Quality Level【质量水平】
200
packaging【包装】
vial of 200 μL
concentration【浓度】
(1x106 TU/ml (via p24 titering assay))
application(s)
CRISPR
shipped in【运输】
dry ice
storage temp.【储存温度】
−70℃
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
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