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Anti- Bovine IgG-Peroxidase antibody, Mouse monoclonal
产品编号: | 4070574 |
规格: | clone BG-18, purified from hybridoma cell culture |
包装规格: | 1 VIAL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti- Bovine IgG-Peroxidase antibody, Mouse monoclonal
Bovine immunoglobulin G
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
conjugate【偶联物】 | peroxidase conjugate |
antibody form【抗体形式】 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
clone【克隆】 | BG-18, monoclonal |
form【形式】 | lyophilized powder |
species reactivity | bovine |
concentration【浓度】 | ~2 mg/mL |
technique(s) | direct ELISA: 1:20,000-1:40,000 using 1 μg/ml bovine IgG for coating |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
基本信息
General description【一般描述】 | Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the BG-18 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with the purified bovine IgG. Immunoglobulin G (IgG) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively. The bovine immunoglobulin system closely resembles that of other mammalian species with respect to the physiochemical properties and nomenclature. The main bovine IgG class antibodies are IgG1 and IgG2, which have antigenic differences in the Fc region of the heavy chains.4 |
Specificity【特异性】 | Monoclonal Anti-Bovine IgG specifically recognizes an epitope on the heavy chain of both bovine IgG1 and IgG2. The antibody shows no cross-reactivity with bovine IgM or IgG from the following species: human, goat, sheep, rabbit, pig, dog, chicken, cat, guinea pig or horse. |
Immunogen【免疫原】 | Purified bovine IgG |
Application【应用】 | The antibody is recommended to use in various immunological techniques, including ELISA1, Immunohistochemistry2 and Immunobloting3 |
Biochem/physiol Actions【生化/生理作用】 | Different immunoglobulin classes and subclasses (isotypes) perform distinctive effector functions. Therefore, the ability to characterize antibody isotype is fundamental to the analysis of the immune response. The diversity of immunoglobulin isotypes associated with the immunity pattern serves as an indicator indistinguishing the phase of infection with various agents. |
Physical form【外形】 | Supplied as a lyophilized powder. |
产品说明
Storage and Stability【储存及稳定性】 | Store the lyophilized product at 2–8 ℃. For extended storage after reconstitution, keep at –20 ℃ in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 ℃ for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Pictograms【象形图】 | GHS07 |
Signal word【警示用语:】 | Warning |
Hazard Statements | H317 |
Precautionary Statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
Hazard Classifications【危险分类】 | Skin Sens. 1 |
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |