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Anti- Bovine IgG-Peroxidase antibody, Mouse monoclonal

产品编号:4070574
规格:clone BG-18, purified from hybridoma cell culture
包装规格:1 VIAL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40705741 VIAL6510
产品别名

Anti- Bovine IgG-Peroxidase antibody, Mouse monoclonal

Bovine immunoglobulin G

产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
conjugate【偶联物】
peroxidase conjugate
antibody form【抗体形式】
purified from hybridoma cell culture
antibody product type
primary antibodies
clone【克隆】
BG-18, monoclonal
form【形式】
lyophilized powder
species reactivity
bovine
concentration【浓度】
~2 mg/mL
technique(s)
direct ELISA: 1:20,000-1:40,000 using 1 μg/ml bovine IgG for coating
isotype【同位素/亚型】
IgG1
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
基本信息
General description【一般描述】
Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the BG-18 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with the purified bovine IgG.
Immunoglobulin G (IgG) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.
The bovine immunoglobulin system closely resembles that of other mammalian species with respect to the physiochemical properties and nomenclature. The main bovine IgG class antibodies are IgG1 and IgG2, which have antigenic differences in the Fc region of the heavy chains.4 
Specificity【特异性】
Monoclonal Anti-Bovine IgG specifically recognizes an epitope on the heavy chain of both bovine IgG1 and IgG2. The antibody shows no cross-reactivity with bovine IgM or IgG from the following species: human, goat, sheep, rabbit, pig, dog, chicken, cat, guinea pig or horse.
Immunogen【免疫原】
Purified bovine IgG
Application【应用】
The antibody is recommended to use in various immunological techniques, including ELISA1, Immunohistochemistry2 and Immunobloting3
Biochem/physiol Actions【生化/生理作用】
Different immunoglobulin classes and subclasses (isotypes) perform distinctive effector functions. Therefore, the ability to characterize antibody isotype is fundamental to the analysis of the immune response. The diversity of immunoglobulin isotypes associated with the immunity pattern serves as an indicator indistinguishing the phase of infection with various agents.
Physical form【外形】
Supplied as a lyophilized powder.
产品说明
Storage and Stability【储存及稳定性】
Store the lyophilized product at 2–8 ℃. For extended storage after reconstitution, keep at –20 ℃ in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 ℃ for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.
Disclaimer【免责声明】
Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Pictograms【象形图】
GHS07
Signal word【警示用语:】
Warning
Hazard Statements
H317
Precautionary Statements
P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
Hazard Classifications【危险分类】
Skin Sens. 1
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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