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Thioredoxin Reductase Kit

产品编号:4069132
规格:sufficient for 100 colorimetric tests
包装规格:1 KIT
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40691321 KIT5730
产品性质
Quality Level【质量水平】
100
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Thioredoxin Reductase Kit|sigma-aldrich
Detection Method
colorimetric
storage temp.【储存温度】
−20℃
suitability【适用性】
The kit is suitable for the measurement of thioredoxin reductase (TrxR) in various samples including tissue, cells and serum.
基本信息
General description【一般描述】
Thioredoxin reductase (TrxR) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.
Features and Benefits【特点和优势】
Thioredoxin Reductase Kit:
  • Simple and fast procedure with results in ∼40 minutes.
Principle【原理】
In the assay TrxR catalyzes the reduction of 5, 5′-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generates a strong yellow color at A412 nm. In crude biological samples, other enzymes such as glutathione reductase and glutathione peroxidase can also reduce DTNB. Therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed. The first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.
安全信息
Pictograms【象形图】
GHS05
Signal word【警示用语:】
Danger
Hazard Statements
H315 - H318
Precautionary Statements
P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
Hazard Classifications【危险分类】
Eye Dam. 1 - Skin Irrit. 2
Storage Class Code【储存分类代码】
10 - Combustible liquids
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