广州伟伯科技有限公司
您的位置:首页 > 产品中心 > Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric)
产品搜索:

Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric)

产品编号:4068335
规格:sufficient for 100 colorimetric tests
包装规格:1 KIT
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40683351 KIT7110
产品性质
Quality Level【质量水平】
100
web metadata keyword.default
Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric)|sigma-aldrich
Detection Method
colorimetric
storage temp.【储存温度】
−20℃
suitability【适用性】
The kit is suitable for the measurement of Phosphoenolpyruvate Carboxykinase activity in various samples including tissue (rat liver, kidney, heart etc.) and adherent/suspension cells (HeLa, Jurkat, HEK 293 cells etc.). As well as the analysis of the gluconeogenesis pathway.
基本信息
General description【一般描述】
Phosphoenolpyruvate Carboxykinase (PEPCK) is an enzyme which belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PEPCK-C, also called PCK1) and mitochondrial form (PEPCK-M). PEPCK-C is a rate-controlling step in gluconeogenesis. Recent studies found abnormal concentrations of PEPCK in diabetic mice. Therefore, accurate measurement of Phosphoenolpyruvate Carboxykinase activity is valuable for both mechanistic and therapeutic studies.
Features and Benefits【特点和优势】
Phosphoenolpyruvate Carboxykinase Activity Assay Kit:
  • Compatible with high-throughput handling systems.
  • Sensitive, detecting than 10 μU of Phosphoenolpyruvate Carboxykinase activity per sample.
Principle【原理】
In this assay, Phosphoenolpyruvate Carboxykinase is coupled with a set of enzymes that convert phosphoenolpyruvate and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and is converted generating a colorimetric signal (A570 nm). The color intensity is directly proportional to the amount of active Phosphoenolpyruvate Carboxykinase present in samples.
安全信息
Pictograms【象形图】
GHS07,GHS08
Signal word【警示用语:】
Danger
Hazard Statements
H317 - H334 - H335
Precautionary Statements
P261 - P271 - P272 - P280 - P284 - P302 + P352
Hazard Classifications【危险分类】
Resp. Sens. 1 - Skin Sens. 1 - STOT SE 3
Target Organs【靶器官】
Respiratory system
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.0160