Anti-Sheep IgG (H+L), F(ab) fragment-Fluorescein antibody produced in donkey

NA.46

Immunoglobulin G (IgG) is sub-divided into IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. It is expressed in the serum and possesses two heavy chains and two light chains. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as Fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Fluorescein and Anti-Donkey Serum. No reaction was observed against Anti-Papain or Anti-Donkey IgG F(c).

Sheep IgG whole molecule

Antibody format: IgG Fab

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitute with 1.0 mL deionized water (or equivalent).

100

donkey

fluorescein conjugate

affinity isolated antibody

secondary antibodies

polyclonal

lyophilized powder

sheep

direct immunofluorescence: suitable
indirect ELISA: suitable
indirect immunofluorescence: suitable

wet ice

2-8℃

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS06,GHS09

Danger

H302 - H311 - H411

P280 - P301 + P312 + P330 - P302 + P352 + P312

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2

EUH032

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK 3