您的位置：首页 > 产品中心 > A549 HLA Panel Cell Lines
A549 HLA Panel Cell Lines
产品性质
| biological source【生物来源】 | human lung (Carcinoma) |
| growth mode【生长模式】 | Adherent |
| morphology【形态学】 | Epithelial |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| General description【一般描述】 | The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 86012804. This product is a panel of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in an A549 lung carcinoma parental cell line background. The full HLA panel consists of a Beta-2 microglobulin (β2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines. CompoZr® Zinc Finger Nuclease (ZFN) technology was used to generate the β2M knockout cell line, which does not express endogenous cell surface MHC Class I HLA molecules (HLA-A or HLA-B) as measured by fluorescence-activated cell sorting (FACS). MISSION® lentivirus was used to generate the ten (10) monoallelic HLA cell lines, which express individual HLA-A or HLA-B subtypes on the cell surface via a β2M:HLA fusion protein as described in Nature Biotechnology 35, 765-772, (2017) and as demonstrated via FACS analysis. |
| Cell Line Description【细胞系描述】 | Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen. |
| Features and Benefits【特点和优势】 | Unlike wild type A549 cells, which express multiple HLA subtypes, the A549 HLA panel cell lines express individual MHC Class I HLA subtypes. These monoallelic HLA cell lines can be used to study peptide binding and presentation in the context of individual HLA subtypes. The panel can also be used to develop cell-based assays to evaluate candidate cancer immunotherapies and identify tumor-specific neoantigens. |
| Components【组分】 | HLA003A-1VL: A549 B2M KO Cells HLA003B-1VL: A549 HLA-A*02:01 Cells HLA003C-1VL: A549 HLA-A*01:01 Cells HLA003D-1VL: A549 HLA-A*03:01 Cells HLA003E-1VL: A549 HLA-A*11:01 Cells HLA003F-1VL: A549 HLA-A*24:02 Cells HLA003G-1VL: A549 HLA-B*15:10 Cells HLA003H-1VL: A549 HLA-B*07:02 Cells HLA003I-1VL: A549 HLA-B*08:01 Cells HLA003J-1VL: A549 HLA-B*35:01 Cells HLA003K-1VL: A549 HLA-B*40:01 Cells |
| Quality【质量】 | Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification. |
| Legal Information【法律信息】 | MISSION Use Licenses Agreement ZFN Use Licenses Agreement CompoZr is a registered trademark of sigma-aldrich Co. LLC MISSION is a registered trademark of sigma-aldrich Co. LLC |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
