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InnoZyme TACE Activity Kit
产品别名
InnoZyme TACE Activity Kit
α-Secretase Activity Kit, TNF-α Converting Enzyme Activity Kit, ADAM17 Activity Kit
ADAM17 Activity Kit, α-Secretase Activity Kit, TNF-α Converting Enzyme Activity Kit
产品性质
Quality Level【质量水平】 | 100 |
usage【用途】 | sufficient for 96 tests |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles protect from light |
assay range | standard curve range: 5-100 ng/mL (as measured with purified recombinant human TACE) |
input | sample type cells |
Detection Method | fluorometric |
shipped in【运输】 | wet ice |
packaging【包装】 | 1 kit in Glass bottle |
基本信息
General description【一般描述】 | The InnoZyme • Highly specific: based on immunocapture of human TACE • Highly sensitive and quantitative: assay range 5-100 ng/ml • Convenient: 96-well format and non-radioactive detection • Versatile: appropriate for measuring active TACE and high-throughput screening of TACE inhibitors |
Components【组分】 | Anti-Human TACE-Coated 96-Well Plate, Control, Substrate, Sample Buffer, Assay Buffer, Wash Buffer, Plate Sealer, and a user protocol. anti-human TACE-coated 96-well plate, TACE control, substrate, sample buffer, assay buffer, wash buffer, plate sealer, user protocol |
Warning【警告】 | Toxicity: Multiple Toxicity Values, refer to MSDS (O) |
Principle【原理】 | The InnoZyme |
Preparation Note【制备说明】 | All reagents necessary to perform the assay are supplied with the kit. Warm all reagents to room temperature (15-25°C) immediately prior to use.For analyzing biological samples the following reagent preparation is required:• Wash Buffer (1X): Dilute Wash Buffer 1:20 with dH2O . To prepare Wash Buffer (1X) for a single wash step for the entire plate, add 2 ml Wash Buffer to 38 ml dH2O. • Control: Dilute the Control with chilled Sample Buffer. Refer to the vial label for lot-specific dilution information.Important Note: Following initial thaw the Control should be dispensed into aliquots and stored at -70°C. Avoid freeze/thaw cycles.• Substrate (1X): Dilute Substrate 1:200 with Assay Buffer. To prepare Substrate (1X) for the entire plate, add 50 µl Substrate to 9.95 ml Assay Buffer.For inhibitor screening the following additional reagent preparation is required:• Test inhibitor(s): Prepare dilutions of test inhibitors in Assay Buffer as necessary.• Diluted Substrate: Dilute Substrate 1:50 with Assay Buffer. To prepare Substrate for the entire plate add 40 µl Substrate to 1.95 ml Assay Buffer. Dilute samples as necessary with Sample Buffer.Recommended guidelines for sample preparation:1. Total protein concentration in cell lysates should be >5 mg/ml2. Dilution factor for cell lysates at >5 mg/ml is approximately 1:20 or higher |
Analysis Note【分析说明】 | The results are displayed in relative fluorescence units (RFU): 1. Correct the fluorescence from each sample and Control by subtracting the fluorescence of the Blank.2. Calculate the mean fluorescence for each sample and Control from duplicate readings to obtain the final RFU. Positive Control Human recombinant TACE |
Other Notes【其他说明】 | Kirkegaard, T., et al. 2004. Clin. Exp. Immunol.135, 146. Neumann, U., et al. 2004. Anal. Biochem.328, 166. Skovronsky, D.M., et al. 2001. J. Neurobiol.49, 40. Black, R.A., et al. 1997. Nature385, 729. Moss, M.L., et al. 1997. Nature385, 733. Selected Citations DasGupta S., et al. 2009. Bioorg. Med. Chem.17, 3604. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品说明
Storage and Stability【储存及稳定性】 | Upon receipt the unopened kit should be stored at -20°C or -70°C. All kit components, once opened, can be stored for up to 3 months under the following conditions: Refrigerate (4°C): Sample Buffer Assay Buffer Wash Buffer Substrate Anti-Human TACE-coated 96-well plate Deep Freeze (-70°C): Control* *Note: Following initial use the Control should be dispensed into aliquots and stored at -70°C. Avoid freeze/thaw cycles. |
安全信息
Storage Class Code【储存分类代码】 | 10-13 - German Storage Class 10 to 13 |