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ToyoScreen® AF-rProtein A HC-650F Process Development Column
| 产品编号： | 3451657 |
| 规格： | phase rProtein A, pkg of 5 × 5 mL, 45 μm particle size |
| 包装规格： | 5 X 5 ML |
| 产品类别： | 进口试剂 |
| 优惠价： | 立即咨询 |
产品性质
| material【物料】 | aluminum seal polypropylene and polyethylene frit |
| product line【产品线】 | ToyoScreen® |
| specific analyte(s) | IgG |
| packaging【包装】 | pkg of 5 × 5 mL |
| manufacturer/tradename | Tosoh 23432 |
| parameter【参数】 | 1-5 mL/min flow rate 20 mL/min max. flow rate 3 bar max. pressure |
| technique(s) | HPLC: suitable |
| L × I.D.【长度 × 内径】 | 3 cm × 14.6 mm |
| matrix【基质】 | hydroxylated methacrylic polymer |
| matrix active group【基质活性基团】 | rProtein A phase |
| Particle size【粒径】 | 45 μm |
| operating pH | 2 - 13 |
| capacity【容量】 | >70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time) >80 g/L adsorption capacity (IgG)(static) |
| separation technique【分离技术】 | affinity |
基本信息
| General description【一般描述】 | Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale. |
| Specifications【产品规格】 | Clean in place with 0.1 M NaOH. |
| Recommended products【推荐产品】 | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| Legal Information【法律信息】 | ToyoScreen is a registered trademark of Tosoh Corporation Toyopearl is a registered trademark of Tosoh Corporation |
