Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr²⁰²/pTyr²⁰⁴), phospho-JNK (pThr¹⁸³/pTyr¹⁸⁵), and phospho-p38 MAPK (pThr¹⁸⁰/pTyr¹⁸²) in cultured cell lines

NA.32

The Cell-Based ERK1/2 (activated) ELISA Sampler Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr²⁰² / pTyr²⁰⁴), JNK (pThr¹⁸³ / pTyr¹⁸⁵) and p38 MAPK (pThr^180m / pTyr¹⁸²) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

ERK1/2 (pThr²⁰² / pTyr²⁰⁴), JNK (pThr¹⁸³ / pTyr¹⁸⁵), p38 MAPK (pThr¹⁸⁰ / pTyr¹⁸²) synthetic phosphopeptides

请参考Protocol了解详情。

组份可单独销售
RABBLOCK 5x Blocking Solution (Item F)
RABFIX1 Fixing Solution (Item D)
RABHRP1 HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)
RABHRP2 HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)
RABPLATE1 Uncoated Microplate
RABQUENCH Quenching Solution for Cell-based ELISA Assay (Item E)
RABSTOP1 Stop Solution
RABTMB1 TMB Substrate Reagent (Item J)
RABWASH1 20X Wash Buffer Concentrate A
RABWASH2 20X Wash Buffer Concentrate B

192 assays

human, mouse, rat

ELISA: suitable
capture ELISA: suitable

wet ice

−20℃

human ... MAPK1(5594), MAPK14(1432), MAPK3(5595), MAPK8(5599)
mouse ... MAPK1(26413), MAPK14(26416), MAPK3(26417), MAPK8(26419)
rat ... MAPK1(116590), MAPK14(81649), MAPK3(50689), MAPK8(116554)

GHS05,GHS07,GHS08

Danger

H290 - H302 + H332 - H315 - H317 - H318 - H335 - H341 - H350 - H412

P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Respiratory system

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects