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CBTF

产品编号:3423736
包装规格:50 MG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
342373650 MG1940
产品别名

CBTF

APN-TFS ester

Sodium 4-((4-(cyanoethynyl)benzoyl)oxy)-2,3,5,6-tetrafluorobenzenesulfonate

结构式
基本信息
Empirical Formula【经验(实验)分子式】
C16H4F4NNaO5S
Molecular weight
421.26
PubChem Substance ID【PubChem化学物质编号】
329768745
NACRES
NA.22
General description【一般描述】
CBTF (Sodium 4-((4-(cyanoethynyl)benzoyl)oxy)-2,3,5,6-tetrafluorobenzenesulfonate) is a heterobifunctional reagent. It bears a 3-arylpropionitrile (APN) group. CBTF can be prepared from 4-iodobenzoic acid. It participates in various amine-to-thiol coupling reactions. It participates in the synthesis of various antibody-dye conjugates. CBTF is widely used for antibody conjugation.
Application【应用】
CBTF is a bifunctional crosslinker for amine-to-thiol coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. CBTF readily reacts with organic amines in basic conditions to produce APN-probes suitable for cysteine labeling.
Other Notes【其他说明】
Standard protein labeling procedure (lysine labeling):
  • Dissolve the protein in an appropriate buffer* with pH 7.5-9.0 (e.g. PBS) at 1-10mg/mL concentration.
  • Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per lysine residue).
  • Incubate at room temperature for 2 hours.
  • If necessary, purify the protein-APN conjugate using size exclusion chromatography or ultrafiltration.
  • The conjugate can be readily coupled with thiol-containing substrates by incubating the components in aqueous buffer (pH 6.5-9.0) at ambient temperature for 2 hours.



Standard protein labeling procedure (cysteine labeling):

Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10mg/mL concentration.
Apply the appropriate amount of the stock solution of the APN-labeled molecule (1-5 molar eq. per free cysteine residue).
Incubate at room temperature for 2 hours.
If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

*Note: avoid amine- and thiol-containing buffers.
产品性质
form【形式】
powder
Quality Level【质量水平】
100
reaction suitability
reagent type: cross-linking reagent
storage temp.【储存温度】
−20℃
SMILES string
O=C(OC1=C(F)C(F)=C(S(=O)(O[Na])=O)C(F)=C1F)C2=CC=C(C#CC#N)C=C2
InChI
1S/C16H5F4NO5S.Na/c17-10-12(19)15(27(23,24)25)13(20)11(18)14(10)26-16(22)9-5-3-8(4-6-9)2-1-7-21;/h3-6H,(H,23,24,25);/q;+1/p-1
InChI key
YFJYSJRZDOWXDH-UHFFFAOYSA-M
packaging【包装】
50 mg in glass bottle
安全信息
Pictograms【象形图】
GHS06
Signal word【警示用语:】
Danger
Hazard Statements
H301
Precautionary Statements
P301 + P310 + P330
Hazard Classifications【危险分类】
Acute Tox. 3 Oral
Storage Class Code【储存分类代码】
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
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