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CBTF
产品别名
CBTF
APN-TFS ester
Sodium 4-((4-(cyanoethynyl)benzoyl)oxy)-2,3,5,6-tetrafluorobenzenesulfonate
结构式
基本信息
Empirical Formula【经验(实验)分子式】 | C16H4F4NNaO5S |
Molecular weight | 421.26 |
PubChem Substance ID【PubChem化学物质编号】 | 329768745 |
NACRES | NA.22 |
General description【一般描述】 | CBTF (Sodium 4-((4-(cyanoethynyl)benzoyl)oxy)-2,3,5,6-tetrafluorobenzenesulfonate) is a heterobifunctional reagent. It bears a 3-arylpropionitrile (APN) group. CBTF can be prepared from 4-iodobenzoic acid. It participates in various amine-to-thiol coupling reactions. It participates in the synthesis of various antibody-dye conjugates. CBTF is widely used for antibody conjugation. |
Application【应用】 | CBTF is a bifunctional crosslinker for amine-to-thiol coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. CBTF readily reacts with organic amines in basic conditions to produce APN-probes suitable for cysteine labeling. |
Other Notes【其他说明】 | Standard protein labeling procedure (lysine labeling):
Standard protein labeling procedure (cysteine labeling): Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10mg/mL concentration. Apply the appropriate amount of the stock solution of the APN-labeled molecule (1-5 molar eq. per free cysteine residue). Incubate at room temperature for 2 hours. If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration. *Note: avoid amine- and thiol-containing buffers. |
产品性质
form【形式】 | powder |
Quality Level【质量水平】 | 100 |
reaction suitability | reagent type: cross-linking reagent |
storage temp.【储存温度】 | −20℃ |
SMILES string | O=C(OC1=C(F)C(F)=C(S(=O)(O[Na])=O)C(F)=C1F)C2=CC=C(C#CC#N)C=C2 |
InChI | 1S/C16H5F4NO5S.Na/c17-10-12(19)15(27(23,24)25)13(20)11(18)14(10)26-16(22)9-5-3-8(4-6-9)2-1-7-21;/h3-6H,(H,23,24,25);/q;+1/p-1 |
InChI key | YFJYSJRZDOWXDH-UHFFFAOYSA-M |
packaging【包装】 | 50 mg in glass bottle |
安全信息
Pictograms【象形图】 | GHS06 |
Signal word【警示用语:】 | Danger |
Hazard Statements | H301 |
Precautionary Statements | P301 + P310 + P330 |
Hazard Classifications【危险分类】 | Acute Tox. 3 Oral |
Storage Class Code【储存分类代码】 | 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects |
WGK | WGK 3 |