您的位置：首页 > 产品中心 > CAD cell line from mouse
产品性质
| biological source【生物来源】 | mouse nerve |
| description【描述】 | Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumor |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Neuronal |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour |
| Cell Line Description【细胞系描述】 | CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions. |
| Application【应用】 | Study of neuronal differentiation |
| Culture Medium【培养基】 | DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS) |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 104 cells/cm2 Cells should be dislodged by gentle pipetting; 5% CO2; 37℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
