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产品性质
biological source【生物来源】 | mouse blood |
growth mode【生长模式】 | Suspension |
morphology【形态学】 | Lymphoblast |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
General description【一般描述】 | For complete product information, please see BW5147.G.1.4.OUA/R.1 |
Cell Line Origin【细胞系来源】 | Mouse AKR/J T cell lymphoma |
Cell Line Description【细胞系描述】 | This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids. |
Application【应用】 | Fusion partner |
Culture Medium【培养基】 | SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Maintain cultures between 1-6x100,000 cells/ml; 5% CO2; 37℃. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |