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亲和素 来源于鸡蛋白

Avidin from egg white
产品编号:3411039
规格:recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)
CAS NO:1405-69-2
包装规格:5 MG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
34110395 MG2910
产品别名

1405-69-2

亲和素 来源于鸡蛋白

Avidin from egg white

基本信息
EC number【EC 号】
215-783-6
MDL number
MFCD00130572
NACRES
NA.56
General description【一般描述】
Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.
Application【应用】
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.
亲和素蛋白用于一项分析中,该分析使用官能化氙作用检测生物素-亲和素结合的生物传感器。使用80nmol的鸡蛋白。
Unit Definition【单位定义】
One unit will bind 1.0 μg of d-biotin.
Preparation Note【制备说明】
亲和纯化
产品性质
Quality Level【质量水平】
200
recombinant【重组】
expressed in corn
Assay【测定】
≥80% protein basis (biuret)
form【形式】
powder
Specific Activity
≥12 units/mg protein (E1%/280)
mol wt【分子量】
glycoprotein 66 kDa
subunit 16 kDa
technique(s)
ELISA: suitable
immunoblotting: suitable
immunoelectrophoresis: suitable
UniProt accession no.【UniProt登记号】
P02701
storage temp.【储存温度】
2-8℃
Gene Information
chicken ... AVD(396260)
packaging【包装】
5 mg in glass bottle
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves, type N95 (US)
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