Anti-SH2B3 antibody produced in rabbit

HPA005483    Human Protein Atlas characterization data

SH2B3 (SH2B adaptor protein 3) is a part of adaptor family of proteins, which are characterized by a conserved tyrosine residue at the C-terminal, an Src homology-2 domain (SH2), a pleckstrin homology domain (PH) and a proline-rich dimerization domain at the N-terminal. It is majorly expressed in hematopoietic tissues and is a membrane bound protein. SH2B3 gene is located on chromosome 12q24, spans 46kbp, contains 9 exons, and encodes for a protein of 575 amino acids.

SH2B adapter protein 3 recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

SH2B3 (SH2B adaptor protein 3) binds to JAK2 and cytokine receptors, via its SH2 domain and inhibits downstream signaling pathway. By interacting with JAK2, it regulates erythropoietin (EPO) and thrombopoietin signaling, and also inhibits STAT pathway. Mutations in this gene are linked with idiopathic erythrocytosis, and mutations in exon 2 are associated with unexplained erythrocytosis, and below normal EPO levels. Different forms of myeloproliferative neoplasms (MPNs), such as primary myelofibrosis (PMF), JAK2V617F-negative erythrocytosis, and blast-phase or chronic MPNs, are associated with various mutations in SH2B3 gene. Studies also show a link between this gene and autoimmune diseases. Coeliac disease is associated with SNP rs3184504 in SH2B3 gene, and there is an increase in the expression of this protein in the intestinal mucosa of coeliac disease patients. There is also a link between SH2B3 and systemic lupus erythematosus, rheumatoid arthritis and thrombophilia.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST86499.

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

rabbit

100

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

Prestige Antibodies^® Powered by Atlas Antibodies

buffered aqueous glycerol solution

human

antibody small pack of 25 μL

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:20-1:50

FDPPKSSRPKLQAACSSIQEVRRCTRLEMPDNLYTFVLKVKDRTDIIFEVGDEQQLNSWMAELSECTGRGLESTEAEMHIPSALEPSTSSSPRGSTDSLNQGASPGGLLDPACQKTDHFLSCYPWFH

Q9UQQ2

wet ice

−20℃

human ... SH2B3(10019)

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10 - Combustible liquids

WGK 1

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)