Anti-Rat IgG (whole molecule)-TRITC antibody produced in rabbit

MFCD00162814

NA.46

IgG is present in large quantities in the human serum. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.
Binds all rat Igs

Anti-Rat IgG (whole molecule)-TRITC antibody has been used in

• confocal microscopy
• immunohistochemistry
• immunofluorescence

IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (Fragment antigen binding) fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Antibody adsorbed with human IgG.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

Adsorbed to reduce background staining with human samples.

rabbit

200

TRITC conjugate

affinity isolated antibody

secondary antibodies

polyclonal

buffered aqueous solution

protect from light

direct immunofluorescence: 1:100 using human peripheral blood
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tonsils

−20℃

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12 - Non Combustible Liquids

WGK 3

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)