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Anti-Raf1 antibody produced in rabbit
产品别名
Anti-Raf1 antibody produced in rabbit
Anti-v-raf-leukemia viral oncogene 1
基本信息
| NACRES | NA.41 |
| General description【一般描述】 | The antibody detects endogenous level of total Raf-1 protein. |
| Immunogen【免疫原】 | Peptide sequence around aa. 641-645 (T-S-P-R-L ), according to the protein NP_036771.1 |
| Features and Benefits【特点和优势】 | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Physical form【外形】 | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
产品性质
| biological source【生物来源】 | rabbit |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | ~74 kDa |
| species reactivity | human, mouse, rat |
| concentration【浓度】 | 1 mg/mL |
| technique(s) | western blot: 1:500-1:1000 |
| isotype【同位素/亚型】 | IgG |
| immunogen sequence【免疫原序列】 | (T-S-P-R-L ) |
| NCBI accession no.【NCBI登记号】 | NM_012639.2 |
| UniProt accession no.【UniProt登记号】 | P04049 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | rat ... Raf1(24703) |
产品说明
| Target description【目标描述】 | A-Raf, B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
